β-PIX is critical for transplanted mesenchymal stromal cell migration

Seong Ho Koh, Yong Min Huh, Min Young Noh, Hyun Young Kim, Kyung Suk Kim, Eun Sook Lee, Hyun Ju Ko, Goang Won Cho, A. Rum Yoo, Ho Taek Song, Sejin Hwang, Kwangyeol Lee, Seungjoo Haam, Joseph A. Frank, Jin Suck Suh, Seung Hyun Kim

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Bone marrow-derived mesenchymal stromal cells (MSCs) have been used successfully as a source of stem cells for treating neurodegenerative diseases. However, for reasons that are not clear, autologous MSC transplants have not yielded successful results in human trials. To test one possible reason, we compared the migratory ability of MSCs from amyotrophic lateral sclerosis (ALS) patients with those of healthy controls. We found that MSCs derived from ALS patients (ALS-MSCs) had a reduced ability to migrate, which may explain why autologous transplantation is not successful. We also found that expression of one of the intracellular factors implicated in migration, β-PIX, was significantly reduced in ALS-MSCs compared with healthy stem cells. Restoration of β-PIX expression by genetic manipulation restored the migratory ability of ALS-MSCs, and inhibition of β-PIX expression with shRNA reduced the migration of healthy MSCs. We suggest that transplantation of allogeneic or genetically modified autologous stem cells might be a more promising strategy for ALS patients than transplantation of autologous stem cells.

    Original languageEnglish
    Pages (from-to)1989-1999
    Number of pages11
    JournalStem cells and development
    Volume21
    Issue number11
    DOIs
    Publication statusPublished - 2012 Jul 20

    ASJC Scopus subject areas

    • Hematology
    • Developmental Biology
    • Cell Biology

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