TY - JOUR
T1 - κ-Carrageenan-essential oil loaded composite biomaterial film facilitates mechanosensing and tissue regenerative wound healing
AU - Sathuvan, Malairaj
AU - Thangam, Ramar
AU - Cheong, Kit Leong
AU - Kang, Heemin
AU - Liu, Yang
N1 - Funding Information:
The Educational Commission of Guangdong Province, China supports this work ( 2021ZDZX2051 ), Li Ka Shing Foundation Cross-Disciplinary Research Grant (Project Numbers: 2020LKSFG02E and 2020LKSFG18B ). The author (R.T.) would like to acknowledge the National Research Foundation of Korea (NRF) grant ( 2021R1I1A1A01046207 ) for funding from the Ministry of Science and Technology , Republic of Korea.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/6/30
Y1 - 2023/6/30
N2 - Polysaccharides κ-carrageenan (κ-Car) have become a predominant source in developing bioactive materials. We aimed to develop biopolymer composite materials of κ-Car with coriander essential oil (CEO) (κ-Car-CEO) films for fibroblast-associated wound healing. Initially, we loaded the CEO in to κ-Car and CEO through homogenization and ultrasonication to fabricate composite film bioactive materials. After performing morphological and chemical characterizations, we validated the developed material functionalities in both in vitro and in vivo models. The chemical and morphological analysis with physical structure, swelling ratio, encapsulation efficiency, CEO release, and water barrier properties of films examined and showed the structural interaction of κ-Car and CEO-loaded into the polymer network. Furthermore, the bioactive applications of CEO release showed initial burst release followed by controlled release from the κ-Car composite film with fibroblast (L929) cell adhesive capabilities and mechanosensing. Our results proved that the CEO-loaded into the κ-Car film impacts cell adhesion, F-actin organization, and collagen synthesis, followed by in vitro mechanosensing activation, further promoting wound healing in vivo. Our innovative perspectives of active polysaccharide (κ-Car)-based CEO functional film materials could potentially accomplish regenerative medicine.
AB - Polysaccharides κ-carrageenan (κ-Car) have become a predominant source in developing bioactive materials. We aimed to develop biopolymer composite materials of κ-Car with coriander essential oil (CEO) (κ-Car-CEO) films for fibroblast-associated wound healing. Initially, we loaded the CEO in to κ-Car and CEO through homogenization and ultrasonication to fabricate composite film bioactive materials. After performing morphological and chemical characterizations, we validated the developed material functionalities in both in vitro and in vivo models. The chemical and morphological analysis with physical structure, swelling ratio, encapsulation efficiency, CEO release, and water barrier properties of films examined and showed the structural interaction of κ-Car and CEO-loaded into the polymer network. Furthermore, the bioactive applications of CEO release showed initial burst release followed by controlled release from the κ-Car composite film with fibroblast (L929) cell adhesive capabilities and mechanosensing. Our results proved that the CEO-loaded into the κ-Car film impacts cell adhesion, F-actin organization, and collagen synthesis, followed by in vitro mechanosensing activation, further promoting wound healing in vivo. Our innovative perspectives of active polysaccharide (κ-Car)-based CEO functional film materials could potentially accomplish regenerative medicine.
KW - Essential oil
KW - Fibroblast cell adhesion
KW - In vivo wound healing
KW - Mechanosensing
KW - Polysaccharides
KW - κ-Carrageenan
UR - http://www.scopus.com/inward/record.url?scp=85153794020&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.124490
DO - 10.1016/j.ijbiomac.2023.124490
M3 - Article
C2 - 37076080
AN - SCOPUS:85153794020
SN - 0141-8130
VL - 241
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 124490
ER -