TY - JOUR
T1 - 2-Methoxyoestradiol levels and placental catechol-O-methyltransferase expression in patients with late-onset preeclampsia
AU - Seol, Hyun Joo
AU - Cho, Geum Joon
AU - Oh, Min Jeong
AU - Kim, Hai Joong
PY - 2013/5
Y1 - 2013/5
N2 - Purpose: Deficiencies in placental catechol-O-methyltransferase (COMT) and circulating 2-methoxyoestradiol (2-ME) have been shown to be related to early onset preeclampsia. The aim of this study was to investigate levels of 2-ME in the serum and urine of patients with late-onset preeclampsia and to compare those levels to those of normal pregnant women. In addition, we examined placental COMT expression in both groups. Methods: Fifteen patients with preeclampsia and 15 normal pregnant women were enrolled. 2-ME levels were evaluated by ELISA and placental COMT expression was examined by Western blot analysis. Results: 2-ME levels in serum [median 181.1 pg/mL, interquartile range (IQR) 119.6-244.3 vs. 61.2 pg/mL, IQR 12.0-133.7, respectively, p = 0.004] and urine (median 143.3 pg/mL, IQR 35.0-328.2 vs. 0.5 pg/mL, IQR 0.4-4.6, respectively, p < 0.001) were significantly increased in patients with late-onset preeclampsia compared to those in normal pregnant women at term. There was no significant difference in placental COMT expression between the two groups. Conclusion: Increased levels of 2-ME in patients with late-onset preeclampsia might be a product of a compensatory mechanism in patients with late-onset preeclampsia.
AB - Purpose: Deficiencies in placental catechol-O-methyltransferase (COMT) and circulating 2-methoxyoestradiol (2-ME) have been shown to be related to early onset preeclampsia. The aim of this study was to investigate levels of 2-ME in the serum and urine of patients with late-onset preeclampsia and to compare those levels to those of normal pregnant women. In addition, we examined placental COMT expression in both groups. Methods: Fifteen patients with preeclampsia and 15 normal pregnant women were enrolled. 2-ME levels were evaluated by ELISA and placental COMT expression was examined by Western blot analysis. Results: 2-ME levels in serum [median 181.1 pg/mL, interquartile range (IQR) 119.6-244.3 vs. 61.2 pg/mL, IQR 12.0-133.7, respectively, p = 0.004] and urine (median 143.3 pg/mL, IQR 35.0-328.2 vs. 0.5 pg/mL, IQR 0.4-4.6, respectively, p < 0.001) were significantly increased in patients with late-onset preeclampsia compared to those in normal pregnant women at term. There was no significant difference in placental COMT expression between the two groups. Conclusion: Increased levels of 2-ME in patients with late-onset preeclampsia might be a product of a compensatory mechanism in patients with late-onset preeclampsia.
KW - 2-Methoxyoestradiol
KW - Catechol-O-methyltransferase
KW - Placenta
KW - Preeclampsia
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U2 - 10.1007/s00404-012-2663-1
DO - 10.1007/s00404-012-2663-1
M3 - Article
C2 - 23233290
AN - SCOPUS:84876691164
SN - 0932-0067
VL - 287
SP - 881
EP - 886
JO - Archives of Gynecology and Obstetrics
JF - Archives of Gynecology and Obstetrics
IS - 5
ER -