2 X 3 array oxygen sensor for measuring cellular respiration level

Jungil Park, Sunyoung Ahn, Youngmi Kim Pak, James Jungho Pak

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    This paper presents a microfabricated 2 × 3 array oxygen sensor integrated with heaters and temperature sensors for measuring cellular respiration level in a solution containing cells. The proposed oxygen sensor consists of 2 x 3 array Clarktype sensors, heaters, and temperature sensors on the substrate. Pt was used for temperature sensors, working electrode and counter electrode of Clark-type sensor because it has very stable physical and chemical properties and resistance linearity to temperature. Ag/AgCI was formed by electrochemical method on patterned Ag and used as reference electrode. Heaters as Au were patterned on the opposite side of the substrate. PDMS was coated on the acrylic plate by using spin coater, and PDMS thickness is about 18pm. It used as an oxygen permeable membrane of Clark-type sensor. The peak oxygen reduction current of the fabricated sensor was measured at -0.8 V by using cyclic voltammetry, and the 90% response time was about 10 sec from full-oxygen state to zero-oxygen state. The fabricated sensor showed good reproducibility for I hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The temperature sensor showed an excellent linearity with a correlation coefficient of 0.999 from 32°C to 55°C.

    Original languageEnglish
    Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
    Pages1054-1057
    Number of pages4
    DOIs
    Publication statusPublished - 2009
    Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
    Duration: 2009 Jan 52009 Jan 8

    Publication series

    Name4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

    Other

    Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
    Country/TerritoryChina
    CityShenzhen
    Period09/1/509/1/8

    Keywords

    • Cellular respiration
    • Clark-type sensor
    • Microfabrication
    • PDMS
    • Potentiostat

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering

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