2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles

Thirupathi Karuppanapandian; Hong Wei Wang; Natarajan Prabakaran; Kandhavelu Jeyalakshmi; Mi Kwon; Kumariah Manoharan; Wook Kim

doi:10.1016/j.plaphy.2010.11.007

2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles

Thirupathi Karuppanapandian, Hong Wei Wang, Natarajan Prabakaran, Kandhavelu Jeyalakshmi, Mi Kwon, Kumariah Manoharan, Wook Kim

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

Leaf senescence induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and senescence inhibition caused by supplementation with silver (Ag⁺) ions in the form of silver nitrate (AgNO₃) or silver nanoparticles (AgNPs) were investigated in 8-day-old mung bean (Vigna radiata L. Wilczek) seedlings. Inhibition of root and shoot elongation were observed in mung bean seedlings treated with 500μM 2,4-D. Concomitantly, the activity of 1-aminocyclopropane-1-carboxylic acid synthase was significantly induced in leaf tissue. Leaf senescence induced by 2,4-D was closely associated with lipid peroxidation as well as increased levels of cytotoxic hydrogen peroxide (H₂O₂) and superoxide radicals (O2·-). Despite decreased catalase activity, the activities of peroxidase, superoxide dismutase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase were increased during 2,4-D-induced leaf senescence. Further, the levels of reduced ascorbate, oxidized ascorbate, and reduced glutathione were markedly decreased, whereas the level of oxidized glutathione increased. 2,4-D-induced leaf senescence in mung bean was accompanied by an increase in positive terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, nuclear DNA fragmentation, and the activity of a 15-kDa Ca²⁺-dependent DNase. Supplementation with 100μM AgNO₃ or AgNPs inhibited 2,4-D-induced leaf senescence. The present results suggest that increased oxidative stress (O2·- and H₂O₂) led to senescence in mung bean leaves. Furthermore, significantly induced antioxidative enzymes are not sufficient to protect mung bean cells from 2,4-D-induced harmful ROS.

Original language	English
Pages (from-to)	168-177
Number of pages	10
Journal	Plant Physiology and Biochemistry
Volume	49
Issue number	2
DOIs	https://doi.org/10.1016/j.plaphy.2010.11.007
Publication status	Published - 2011 Feb

Bibliographical note

Funding Information:
The first author (TK) expresses his sincere thanks to Dr. J. Rajendhran, Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, for helpful discussion and critical review of this manuscript. Statistical analysis support from Mrs. Sathiya Karuppanapandian, is thankfully acknowledged. This work was financially supported by grants from University Grants Commission under Centre for Excellence in Genomic Sciences (F.14-2/99-IUC/Univ. Excellence) and a Korea University Grant. We also wish to thank the anonymous reviewers for their comments and suggestions to improve our manuscript on various levels.

Keywords

2,4-Dichlorophenoxyacetic acid
Ethylene
Leaf senescence
Mung bean
Nuclear DNA fragmentation
Silver nanoparticles

ASJC Scopus subject areas

Physiology
Genetics
Plant Science

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10.1016/j.plaphy.2010.11.007

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Karuppanapandian, T., Wang, H. W., Prabakaran, N., Jeyalakshmi, K., Kwon, M., Manoharan, K., & Kim, W. (2011). 2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles. Plant Physiology and Biochemistry, 49(2), 168-177. https://doi.org/10.1016/j.plaphy.2010.11.007

2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles. / Karuppanapandian, Thirupathi; Wang, Hong Wei; Prabakaran, Natarajan et al.
In: Plant Physiology and Biochemistry, Vol. 49, No. 2, 02.2011, p. 168-177.

Research output: Contribution to journal › Article › peer-review

Karuppanapandian, T, Wang, HW, Prabakaran, N, Jeyalakshmi, K, Kwon, M, Manoharan, K & Kim, W 2011, '2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles', Plant Physiology and Biochemistry, vol. 49, no. 2, pp. 168-177. https://doi.org/10.1016/j.plaphy.2010.11.007

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abstract = "Leaf senescence induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and senescence inhibition caused by supplementation with silver (Ag+) ions in the form of silver nitrate (AgNO3) or silver nanoparticles (AgNPs) were investigated in 8-day-old mung bean (Vigna radiata L. Wilczek) seedlings. Inhibition of root and shoot elongation were observed in mung bean seedlings treated with 500μM 2,4-D. Concomitantly, the activity of 1-aminocyclopropane-1-carboxylic acid synthase was significantly induced in leaf tissue. Leaf senescence induced by 2,4-D was closely associated with lipid peroxidation as well as increased levels of cytotoxic hydrogen peroxide (H2O2) and superoxide radicals (O2·-). Despite decreased catalase activity, the activities of peroxidase, superoxide dismutase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase were increased during 2,4-D-induced leaf senescence. Further, the levels of reduced ascorbate, oxidized ascorbate, and reduced glutathione were markedly decreased, whereas the level of oxidized glutathione increased. 2,4-D-induced leaf senescence in mung bean was accompanied by an increase in positive terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, nuclear DNA fragmentation, and the activity of a 15-kDa Ca2+-dependent DNase. Supplementation with 100μM AgNO3 or AgNPs inhibited 2,4-D-induced leaf senescence. The present results suggest that increased oxidative stress (O2·- and H2O2) led to senescence in mung bean leaves. Furthermore, significantly induced antioxidative enzymes are not sufficient to protect mung bean cells from 2,4-D-induced harmful ROS.",

keywords = "2,4-Dichlorophenoxyacetic acid, Ethylene, Leaf senescence, Mung bean, Nuclear DNA fragmentation, Silver nanoparticles",

author = "Thirupathi Karuppanapandian and Wang, {Hong Wei} and Natarajan Prabakaran and Kandhavelu Jeyalakshmi and Mi Kwon and Kumariah Manoharan and Wook Kim",

note = "Funding Information: The first author (TK) expresses his sincere thanks to Dr. J. Rajendhran, Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, for helpful discussion and critical review of this manuscript. Statistical analysis support from Mrs. Sathiya Karuppanapandian, is thankfully acknowledged. This work was financially supported by grants from University Grants Commission under Centre for Excellence in Genomic Sciences (F.14-2/99-IUC/Univ. Excellence) and a Korea University Grant. We also wish to thank the anonymous reviewers for their comments and suggestions to improve our manuscript on various levels.",

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AU - Karuppanapandian, Thirupathi

AU - Wang, Hong Wei

AU - Prabakaran, Natarajan

AU - Jeyalakshmi, Kandhavelu

AU - Kwon, Mi

AU - Manoharan, Kumariah

AU - Kim, Wook

N1 - Funding Information: The first author (TK) expresses his sincere thanks to Dr. J. Rajendhran, Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, for helpful discussion and critical review of this manuscript. Statistical analysis support from Mrs. Sathiya Karuppanapandian, is thankfully acknowledged. This work was financially supported by grants from University Grants Commission under Centre for Excellence in Genomic Sciences (F.14-2/99-IUC/Univ. Excellence) and a Korea University Grant. We also wish to thank the anonymous reviewers for their comments and suggestions to improve our manuscript on various levels.

PY - 2011/2

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N2 - Leaf senescence induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and senescence inhibition caused by supplementation with silver (Ag+) ions in the form of silver nitrate (AgNO3) or silver nanoparticles (AgNPs) were investigated in 8-day-old mung bean (Vigna radiata L. Wilczek) seedlings. Inhibition of root and shoot elongation were observed in mung bean seedlings treated with 500μM 2,4-D. Concomitantly, the activity of 1-aminocyclopropane-1-carboxylic acid synthase was significantly induced in leaf tissue. Leaf senescence induced by 2,4-D was closely associated with lipid peroxidation as well as increased levels of cytotoxic hydrogen peroxide (H2O2) and superoxide radicals (O2·-). Despite decreased catalase activity, the activities of peroxidase, superoxide dismutase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase were increased during 2,4-D-induced leaf senescence. Further, the levels of reduced ascorbate, oxidized ascorbate, and reduced glutathione were markedly decreased, whereas the level of oxidized glutathione increased. 2,4-D-induced leaf senescence in mung bean was accompanied by an increase in positive terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, nuclear DNA fragmentation, and the activity of a 15-kDa Ca2+-dependent DNase. Supplementation with 100μM AgNO3 or AgNPs inhibited 2,4-D-induced leaf senescence. The present results suggest that increased oxidative stress (O2·- and H2O2) led to senescence in mung bean leaves. Furthermore, significantly induced antioxidative enzymes are not sufficient to protect mung bean cells from 2,4-D-induced harmful ROS.

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2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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