Abstract
In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.
Original language | English |
---|---|
Pages (from-to) | 1055-1060 |
Number of pages | 6 |
Journal | Biotechnology Progress |
Volume | 28 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2012 Jul |
Externally published | Yes |
Keywords
- 3,4-dihydroxy-L-phenylalanine
- Cell adhesion
- Cell adhesion molecule
- Dermal fibroblasts
- Serum-free culture
ASJC Scopus subject areas
- Biotechnology