TY - GEN
T1 - 3d culture strategy to enhance hair inductive potential of human hair follicle derived dermal papilla cells
AU - Choi, Seongkyun
AU - Ahn, Jinchul
AU - Yang, Ji Hun
AU - Chung, Seok
N1 - Funding Information:
This work was supported by the Technology Innovation Program (or Industrial Strategic Technology Development Program(10067796, Development of Genomic-Clinical Data integrated Organoid BioBank System of Korean 5 major cancers and Medical engineering devices for Precision cancer medicine) funded By the Ministry of Trade, industry & Energy(MI, Korea).
Funding Information:
This work was supported by the Technology Innovation Program (or Industrial Strategic Technology Development Program(10067796, Development of Genomic-Clinical Data integrated Organoid BioBank System of Korean 5 major cancers and Medical engineering devices for Precision cancer medicine) funded By the Ministry of Trade, industry & Energy(MI, Korea).
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - The dermal papilla is a cluster of specially aggregated mesenchymal cells located beneath the hair follicle(HF) which plays a crucial role in hair morphogenesis and cycle. Therefore, dermal papilla(DP) is a very important source for researches on hair. However, isolated DP cells(DPCs) easily lost their hair inductive potential within several subcultures. Here, we suggest a simple culture method for DPCs in 3D using a concave type plate and maintain hair inductive potential, without any treatment. Alkaline phosphatase (ALP) were examined to investigate 3D cultured DP spheroids and can sustain the hair inductive potential, which was rarely observed in 2D culture.
AB - The dermal papilla is a cluster of specially aggregated mesenchymal cells located beneath the hair follicle(HF) which plays a crucial role in hair morphogenesis and cycle. Therefore, dermal papilla(DP) is a very important source for researches on hair. However, isolated DP cells(DPCs) easily lost their hair inductive potential within several subcultures. Here, we suggest a simple culture method for DPCs in 3D using a concave type plate and maintain hair inductive potential, without any treatment. Alkaline phosphatase (ALP) were examined to investigate 3D cultured DP spheroids and can sustain the hair inductive potential, which was rarely observed in 2D culture.
KW - 3D culture
KW - Dermal Papilla
KW - Hair follicle
KW - Hair inductive potential
UR - http://www.scopus.com/inward/record.url?scp=85094974412&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094974412
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 1498
EP - 1499
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -