Abstract
The aim of this study was to evaluate the in vitro osteogenic effects and in vivo new bone formation of three-dimensional (3D) printed alendronate (Aln)-releasing poly(caprolactone) (PCL) (Aln/PCL) scaffolds in rat tibial defect models. 3D printed Aln/PCL scaffolds were fabricated via layer-by-layer deposition. The fabricated Aln/PCL scaffolds had high porosity and an interconnected pore structure and showed sustained Aln release. In vitro studies showed that MG-63 cells seeded on the Aln/PCL scaffolds displayed increased alkaline phosphatase (ALP) activity and calcium content in a dose-dependent manner when compared with cell cultures in PCL scaffolds. In addition, in vivo animal studies and histologic evaluation showed that Aln/PCL scaffolds implanted in a rat tibial defect model markedly increased new bone formation and mineralized bone tissues in a dose-dependent manner compared to PCL-only scaffolds. Our results show that 3D printed Aln/PCL scaffolds are promising templates for bone tissue engineering applications.
| Original language | English |
|---|---|
| Article number | 055005 |
| Journal | Biomedical Materials (Bristol) |
| Volume | 11 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2016 Sept 29 |
Bibliographical note
Publisher Copyright:© 2016 IOP Publishing Ltd.
Keywords
- MG-63 cells
- alendronate
- bone formation
- three-dimensional (3D) printed scaffold
- tibial defect model
ASJC Scopus subject areas
- Bioengineering
- Biomaterials
- Biomedical Engineering