5,7-Dimethoxyflavone induces apoptotic cell death in human endometriosis cell lines by activating the endoplasmic reticulum stress pathway

Wonhyoung Park, Min Young Park, Gwonhwa Song, Whasun Lim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Endometriosis is a reproductive disorder characterized by the dislocation of endometrial tissues. Approximately 5–20% of women at their reproductive age are diagnosed with endometriosis, which causes chronic pain and infertility. Here, we demonstrated that the bioactive flavonoid, 5,7-dimethoxyflavone (DMF), exhibited antiproliferative and apoptotic effects in VK2/E6E7 and End1/E6E7 cells which were established from vaginal and endocervical tissue taken from a premenopausal woman undergoing hysterectomy for endometriosis. DMF treatment significantly elevated DNA fragmentation resulting in apoptotic cell death in both cell lines. Furthermore, DMF induced loss of mitochondrial membrane potential, dysregulation of intracellular calcium level, and ROS production, which accelerate apoptosis. Additionally, DMF modulated the expression of the signaling molecules related to cell survival and endoplasmic reticulum stress in VK2/E6E7 and End1/E6E7 cells. Overall, DMF may ameliorate endometriosis and can be a potential alternative to hormonal and surgical therapy for endometriosis treatment.

Original languageEnglish
Pages (from-to)2275-2286
Number of pages12
JournalPhytotherapy Research
Volume34
Issue number9
DOIs
Publication statusPublished - 2020 Sept 1

Bibliographical note

Funding Information:
information: The Korea Health Technology R&D Project through the National Research Foundation of Korea (NRF) grant (grant number: 2018R1C1B6009048)This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare (grant number: HI17C0929) and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT(MSIT) (No. 2018R1C1B6009048).

Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare (grant number: HI17C0929) and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT(MSIT) (No. 2018R1C1B6009048).

Funding Information:
The National Research Foundation of Korea(NRF) grant, Grant/Award Number: 2018R1C1B6009048 Funding information

Publisher Copyright:
© 2020 John Wiley & Sons, Ltd.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • 5,7-Dimethoxyflavone
  • apoptosis
  • endometriosis
  • toxicology

ASJC Scopus subject areas

  • Pharmacology

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