7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates β-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus

Chakradhar Dasagrandhi, Young Soon Kim, In Hwan Kim, Ching T. Hou, Hak Ryul Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infections with multi-drug resistance needs effective and alternative control strategies. In this study we investigated the adjuvant effect of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid (7,10-EODA) against multidrug-resistant S. aureus (MDRSA) strain 01ST001 by disc diffusion, checker board and time kill assays. Further the membrane targeting action of 7,10-EODA was investigated by spectroscopic and confocal microscopic studies. 7,10-EODA exerted synergistic activity along with β-lactam antibiotics against all clinical MRSA strains, with a mean fractional inhibitory concentration index below 0.5. In time-kill kinetic study, combination of 7,10-EODA with oxacillin, ampicillin, and penicillin resulted in 3.8–4.2 log10 reduction in the viable counts of MDRSA 01ST001. Further, 7,10-EODA dose dependently altered the membrane integrity (p < 0.001) and increased the binding of fluorescent analog of penicillin, Bocillin-FL to the MDRSA cells. The membrane action of 7,10-EODA further facilitated the uptake of several other antibiotics in MDRSA. The results of the present study suggested that 7,10-EODA could be a novel antibiotic adjuvant, especially useful in repurposing β-lactam antibiotics against multidrug-resistant MRSA.

Original languageEnglish
Pages (from-to)461-469
Number of pages9
JournalIndian Journal of Microbiology
Volume57
Issue number4
DOIs
Publication statusPublished - 2017 Dec 1

Bibliographical note

Funding Information:
Acknowledgements This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and future planning (2015R1A2A2A01005656).

Publisher Copyright:
© 2017, Association of Microbiologists of India.

Keywords

  • 7,10-Epoxyoctadeca-7,9-dienoic acid
  • Furan fatty acids
  • Multidrug-resistant Staphylococcus aureus
  • Synergistic antibacterial agent
  • β-Lactam antibiotics

ASJC Scopus subject areas

  • Microbiology

Fingerprint

Dive into the research topics of '7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates β-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus'. Together they form a unique fingerprint.

Cite this