A bio-inspired highly selective enzymatic glucose sensor using a red blood cell membrane

Insu Kim, Chaeyeon Kim, Dongtak Lee, Sang Won Lee, Gyudo Lee, Dae Sung Yoon

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    In the development of enzymatic glucose sensors, accurate glucose sensing has been a challenging task because of the existence of numerous interfering molecules in the blood. Meanwhile, red blood cells (RBCs) selectively uptake glucose via a membrane protein called glucose transporter-1. In this study, we developed the RBC membrane (RBCM)-coated enzymatic glucose sensors that mimic the glucose uptake. The RBCM-coated sensors were examined via scanning electron microscopy, atomic force microscopy, and ATR-FTIR. We optimized the glucose permeability of the RBCM filter by controlling the thickness of the filter. The sensing range of the optimized sensor was 1-15 mM, the detection limit was 0.66 mM, and the sensitivity was 2.978 μA mM-1. Intriguingly, the RBCM-coated sensor was highly accurate and precise, despite the coexistence of glucose and interfering molecules (e.g., mannose, galactose, ascorbic acid, uric acid, and cysteine). For each interfering molecule, the errors of our sensor were 0.8 to 2.3%, which was 4.8-14.2 times more accurate than the uncoated one. A similar result was verified for a human serum sample containing countless interfering molecules. Also, the sensing performance of the sensor was consistent after 4 weeks of storage. The results suggest that applying RBCM may improve the selectivity of various types of glucose sensors including the continuous monitoring system.

    Original languageEnglish
    Pages (from-to)2125-2132
    Number of pages8
    JournalAnalyst
    Volume145
    Issue number6
    DOIs
    Publication statusPublished - 2020 Mar 21

    Bibliographical note

    Funding Information:
    This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. NRF-2019R1A2B5B01070617, NRF-2017R1A6A3A11034311, and NRF-2018M3C1B7020722). This research was also supported by the Korea University Graduate School Junior Fellow Research Grant.

    Publisher Copyright:
    © 2020 The Royal Society of Chemistry.

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy
    • Electrochemistry

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