Abstract
A whole-cell biosensor was developed for the detection of gas toxicity using a recombinant bioluminescent Escherichia coli harboring a lac::luxCDABE fusion. Immobilization of the cells within LB agar has been done to maintain the activity of the microorganisms and to detect the toxicity of chemicals through the direct contact with gas. Benzene, known as a representative volatile organic compound, was chosen as a sample toxic gas to evaluate the performance of this biosensor based on the bioluminescent response. This biosensor showed a dose-dependent response, and was found to be reproducible. The immobilizing matrices of this biosensor were stored at 4°C and were maintained for at least a month without any noticible change in its activity. The optimal temperature for sensing was 37°C. A small size of this sensor kit has been successfully fabricated, and found to be applicable as a disposable and portable biosensor to monitor the atmospheric environment of a workplace in which high concentrations of toxic gases could be discharged.
| Original language | English |
|---|---|
| Pages (from-to) | 23-30 |
| Number of pages | 8 |
| Journal | Biosensors and Bioelectronics |
| Volume | 15 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2000 |
| Externally published | Yes |
Bibliographical note
Funding Information:Authors wish to thank Dr Marincs for kindly sending the plasmid pLITE2 for this study. This research was funded from the Center of Excellence of ADEMRC supported by Korea Science and Engineering Foundation (KOSEF), and authors are grateful for the support.
Keywords
- Benzene
- Gas toxicity
- Recombinant bioluminescent Escherichia coli
- Whole cell biosensor
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry