A brief guide to isolating primary hepatocytes from mice

Jisoo Song; Garam An; Gwonhwa Song; Wei Ying; Whasun Lim

doi:10.1016/j.mocell.2026.100330

A brief guide to isolating primary hepatocytes from mice

Jisoo Song
, Garam An
, Gwonhwa Song
, Wei Ying
, Whasun Lim^*

^*Corresponding author for this work

Department of Biotechnology

Research output: Contribution to journal › Letter › peer-review

Abstract

The isolation of primary hepatocytes, which are liver parenchymal cells, is a valuable tool in biomedical research for exploring liver physiology and disease mechanisms. However, obtaining hepatocytes with high viability can be challenging owing to the technical complexity of the procedure and fragility of the cells. Here, we present an optimized protocol, along with key considerations, for isolating hepatocytes from mice with high yield and viability, thereby contributing to liver–related biomedical research.

Original language	English
Article number	100330
Journal	Molecules and cells
Volume	49
Issue number	4
DOIs	https://doi.org/10.1016/j.mocell.2026.100330
Publication status	Published - 2026 Apr

Bibliographical note

Publisher Copyright:
© 2026 The Author(s).

Keywords

Cell culture
Liver
Liver perfusion
Mouse
Primary hepatocyte

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.mocell.2026.100330

Cite this

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AU - Ying, Wei

AU - Lim, Whasun

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N2 - The isolation of primary hepatocytes, which are liver parenchymal cells, is a valuable tool in biomedical research for exploring liver physiology and disease mechanisms. However, obtaining hepatocytes with high viability can be challenging owing to the technical complexity of the procedure and fragility of the cells. Here, we present an optimized protocol, along with key considerations, for isolating hepatocytes from mice with high yield and viability, thereby contributing to liver–related biomedical research.

AB - The isolation of primary hepatocytes, which are liver parenchymal cells, is a valuable tool in biomedical research for exploring liver physiology and disease mechanisms. However, obtaining hepatocytes with high viability can be challenging owing to the technical complexity of the procedure and fragility of the cells. Here, we present an optimized protocol, along with key considerations, for isolating hepatocytes from mice with high yield and viability, thereby contributing to liver–related biomedical research.

KW - Cell culture

KW - Liver

KW - Liver perfusion

KW - Mouse

KW - Primary hepatocyte

UR - https://www.scopus.com/pages/publications/105030926195

U2 - 10.1016/j.mocell.2026.100330

DO - 10.1016/j.mocell.2026.100330

M3 - Letter

C2 - 41679470

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ER -

A brief guide to isolating primary hepatocytes from mice

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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