A calix[2]triazole[2]arene-based fluorescent chemosensor for probing the copper trafficking pathway in Wilson's disease

Jihee Cho, Tuhin Pradhan, Yun Mi Lee, Jong Seung Kim, Sanghee Kim

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    A pyrenyl-appended calix[2]triazole[2]arene displaying excellent selectivity for Cu2+ over other metal ions in pyrenyl excimer emission changes was synthesized. The binding mode with Cu2+ was supported through nuclear magnetic resonance (NMR) studies and density functional theory (DFT) calculations. Fluorescence imaging demonstrates that this new copper sensor is capable of detecting intracellular copper in living cells. Furthermore, through colocalization of the probe with organelle trackers as a function of time, it was observed that copper initially accumulates in lysosomes and then decreases. These results provide evidence for a close relationship between copper and lysosomes in Wilson's disease. This system is the first Cu2+ ion-induced fluorescent turn-on system used for imaging copper trafficking over time in a Wilson's disease model.

    Original languageEnglish
    Pages (from-to)16178-16182
    Number of pages5
    JournalDalton Transactions
    Volume43
    Issue number43
    DOIs
    Publication statusPublished - 2014 Nov 21

    Bibliographical note

    Publisher Copyright:
    © 2014 the Partner Organisations.

    ASJC Scopus subject areas

    • Inorganic Chemistry

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