A cathepsin F of adult Clonorchis sinensis and its phylogenetic conservation in trematodes

T. H. Kang, D. H. Yun, E. H.B. Lee, Y. B. Chung, Y. A. Bae, J. Y. Chung, I. Kang, J. Kim, S. Y. Cho, Y. Kong

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


A novel 28 kDa cysteine protease (Cs28CF) secreted by the hepatobiliary trematode, Clonorchis sinensis was identified. The protease was purified from the excretory-secretory products (ESP) of the adult worm using DEAE-ion exchange and Arginine-Sepharose 4B chromatography. It showed a high activity between pH 6.5 and 7.5 in a dithiothreitol (DTT)-dependent manner. Inhibitors specific to cysteine proteases down-regulated the activity. Addition of Cs28CF to monkey cholangiocyte cultures resulted in approximately 95% cell death after 7 days. The full-length cDNA (1078 bp) encoded a single peptide of 328 amino acids (aa) with an N-terminal hydrophobic sequence, an ERFNAQ motif in the propeptide and a mature domain. Expression of mRNA transcripts of Cs28CF was observed in both the metacercaria and adult stages. Bacterially expressed recombinant protein exhibited a specific antibody reaction with clonorchiasis sera. Deduced aa exhibited 52-76% sequence identity with the cathepsin F analogues from other organisms. A novel E/DXGTA motif was recognized in the propeptide region. Phylogenetic analysis of 63 papain family members revealed that the trematode cysteine proteases formed 2 major clades of cathepsins F and L. The trematode cysteine proteases classified as cathepsin F shared higher homology among themselves than those classified as cathepsin L. Cathepsin F is phylogenetically conserved in the trematode parasites as well as in mammals.

Original languageEnglish
Pages (from-to)195-207
Number of pages13
Issue number2
Publication statusPublished - 2004 Feb


  • Cathepsin F
  • Clonorchis sinensis
  • E/DXGTA motif
  • ERFNAQ motif
  • Excretory-secretory cysteine protease
  • Recombinant antigen

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases


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