We have developed a trifunctional cleavable fluorescence turn-ON linker for chemoproteomic applications. This novel linker, which became highly fluorescent only upon cleavage of the azo bond, was successfully used for in situ proteome profiling/target identification and studies on newly synthesised proteomes.
Bibliographical noteFunding Information:
Funding was provided by NMRC (CBRG/0038/2013), GSK-EDB Trust Fund (R-143-000-688-592), MOE-T1 (R-143-000-694-114) of Singapore, KIST (2E26110/CAP-16-02-KIST) and the Bio & Medical Technology Development Program of the National Research Foundation funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3A9B6902060, NRF-2017M3A9D8029942) of Korea the and National Science Foundation of Zhejiang Province (LQ16B020003).
© 2017 The Royal Society of Chemistry.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry