A clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing

Seungchan Cho; Jon C. Allison; Kkothanahreum Park; Jin Sun No; Seung Ho Lee; Kyungmin Park; Jongwoo Kim; Terry A. Klein; Heung Chul Kim; Won Keun Kim; Jin Won Song

doi:10.3390/pathogens10040424

A clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing

Seungchan Cho, Jon C. Allison, Kkothanahreum Park, Jin Sun No, Seung Ho Lee, Kyungmin Park, Jongwoo Kim, Terry A. Klein, Heung Chul Kim, Won Keun Kim, Jin Won Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain.

Original language	English
Article number	424
Journal	Pathogens
Volume	10
Issue number	4
DOIs	https://doi.org/10.3390/pathogens10040424
Publication status	Published - 2021 Apr

Bibliographical note

Funding Information:
This study was supported by the Agency for Defense Development (South Korea) (grant number: UE202026GD). Partial funding was provided by the Armed Forces Health Surveillance Branch, Global Emerging Infections Surveillance and Response System (AFHSB-GEIS), Silver Spring, MD (ProMIS ID #P0025-2016-2018-ME) and the 65th Medical Brigade, Camp Humphreys, Republic of Korea. The opinions expressed herein are those of the authors and are not to be construed as official or reflecting the views of the U.S. Department of the Army or Defense. Authors, as employees of the U.S. Government (J.C.A., T.A.K., H.-C.K.), conducted the work as part of their official duties. Title 17 U.S.C. §105 provides that ‘Copyright protection under this title is not available for any work of the United States Government.’ ‘Title 17 U.S.C. §101 defines U.S. Government work as work prepared by an employee of the U.S. Government as part of the person’s official duties.’.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Genetic identification
Orientia tsutsugamushi
Scrub typhus
Target enrichment nextgeneration sequencing

ASJC Scopus subject areas

Immunology and Allergy
Molecular Biology
General Immunology and Microbiology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/pathogens10040424

Cite this

Cho, S., Allison, J. C., Park, K., No, J. S., Lee, S. H., Park, K., Kim, J., Klein, T. A., Kim, H. C., Kim, W. K., & Song, J. W. (2021). A clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing. Pathogens, 10(4), Article 424. https://doi.org/10.3390/pathogens10040424

Cho, S, Allison, JC, Park, K, No, JS, Lee, SH, Park, K, Kim, J, Klein, TA, Kim, HC, Kim, WK & Song, JW 2021, 'A clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing', Pathogens, vol. 10, no. 4, 424. https://doi.org/10.3390/pathogens10040424

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abstract = "An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain.",

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author = "Seungchan Cho and Allison, {Jon C.} and Kkothanahreum Park and No, {Jin Sun} and Lee, {Seung Ho} and Kyungmin Park and Jongwoo Kim and Klein, {Terry A.} and Kim, {Heung Chul} and Kim, {Won Keun} and Song, {Jin Won}",

note = "Funding Information: This study was supported by the Agency for Defense Development (South Korea) (grant number: UE202026GD). Partial funding was provided by the Armed Forces Health Surveillance Branch, Global Emerging Infections Surveillance and Response System (AFHSB-GEIS), Silver Spring, MD (ProMIS ID #P0025-2016-2018-ME) and the 65th Medical Brigade, Camp Humphreys, Republic of Korea. The opinions expressed herein are those of the authors and are not to be construed as official or reflecting the views of the U.S. Department of the Army or Defense. Authors, as employees of the U.S. Government (J.C.A., T.A.K., H.-C.K.), conducted the work as part of their official duties. Title 17 U.S.C. §105 provides that {\textquoteleft}Copyright protection under this title is not available for any work of the United States Government.{\textquoteright} {\textquoteleft}Title 17 U.S.C. §101 defines U.S. Government work as work prepared by an employee of the U.S. Government as part of the person{\textquoteright}s official duties.{\textquoteright}. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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A clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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