A clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of orientia tsutsugamushi using multiplex pcr-based next-generation sequencing

Seungchan Cho, Jon C. Allison, Kkothanahreum Park, Jin Sun No, Seung Ho Lee, Kyungmin Park, Jongwoo Kim, Terry A. Klein, Heung Chul Kim, Won Keun Kim, Jin Won Song

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain.

Original languageEnglish
Article number424
JournalPathogens
Volume10
Issue number4
DOIs
Publication statusPublished - 2021 Apr

Bibliographical note

Funding Information:
This study was supported by the Agency for Defense Development (South Korea) (grant number: UE202026GD). Partial funding was provided by the Armed Forces Health Surveillance Branch, Global Emerging Infections Surveillance and Response System (AFHSB-GEIS), Silver Spring, MD (ProMIS ID #P0025-2016-2018-ME) and the 65th Medical Brigade, Camp Humphreys, Republic of Korea. The opinions expressed herein are those of the authors and are not to be construed as official or reflecting the views of the U.S. Department of the Army or Defense. Authors, as employees of the U.S. Government (J.C.A., T.A.K., H.-C.K.), conducted the work as part of their official duties. Title 17 U.S.C. §105 provides that ‘Copyright protection under this title is not available for any work of the United States Government.’ ‘Title 17 U.S.C. §101 defines U.S. Government work as work prepared by an employee of the U.S. Government as part of the person’s official duties.’.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Genetic identification
  • Orientia tsutsugamushi
  • Scrub typhus
  • Target enrichment nextgeneration sequencing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • General Immunology and Microbiology
  • Microbiology (medical)
  • Infectious Diseases

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