A comparative study of analytical methods for alkali-soluble β-glucan in medicinal mushroom, Chaga (Inonotus obliquus)

Sook Jong Rhee, Seung Yong Cho, Ki Myong Kim, Dong Su Cha, Hyun Jin Park

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Crude β-glucan content in the medicinal mushroom Chaga (Inonotus obliquus) was measured by different extraction and analytical methods, and the results were compared. The alkali extraction (AE) method or enzymatic digestion (ED) method followed by a gravimetric analysis was employed to determine the crude β-glucan content. The amount of crude β-glucan in Chaga obtained by either AE or ED was 13.7 g/100 g or 15.3 g/100 g of the sample, respectively. Crude β-glucan content of Chaga obtained by the above preparation methods were corrected by chemical composition analysis and HPLC analysis. After composition analysis, the amounts of β-glucan measured in the 100 g Chaga samples were corrected to 10.1 g for ED and 10.7 g for AE method. β-glucan contents calculated by the amount of glucose in the HPLC analysis were 8.3 g/100 g and 8.1 g/100 g for ED and AE preparation methods, respectively. Although extraction method did not affect β-glucan content in Chaga as indicated by no significant difference (P>0.05) between extraction method, significant differences (P<0.05) were noted between the correction methods. The discrepancies of the result indicate a need for standardization of analytical method for β-glucan measurement in Chaga.

Original languageEnglish
Pages (from-to)545-549
Number of pages5
Issue number3
Publication statusPublished - 2008 Apr

Bibliographical note

Funding Information:
This work was supported by a grant (07062KFDA104) from Korea Food & Drug Administration in 2007, and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2004-216-F00232).


  • Analytical method
  • Chaga
  • Inonotus obliquus
  • Medicinal mushroom
  • Standardization
  • β-glucan

ASJC Scopus subject areas

  • Food Science


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