A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture

Seung Kwan Moon; Myeong Jin Oh; Dong Hyun Paik; Tae Kyung Ryu; Kyeongsoon Park; Sung Eun Kim; Jong Hoon Park; Jung Hyun Kim; Sung Wook Choi

doi:10.1002/marc.201200711

A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture

Seung Kwan Moon, Myeong Jin Oh, Dong Hyun Paik, Tae Kyung Ryu, Kyeongsoon Park, Sung Eun Kim, Jong Hoon Park, Jung Hyun Kim, Sung Wook Choi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.

Original language	English
Pages (from-to)	399-405
Number of pages	7
Journal	Macromolecular Rapid Communications
Volume	34
Issue number	5
DOIs	https://doi.org/10.1002/marc.201200711
Publication status	Published - 2013 Mar 12
Externally published	Yes

Keywords

cell culture
fluidic devices
monodisperse beads
porous materials
uniform pore size

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1002/marc.201200711

Cite this

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abstract = "This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.",

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AU - Moon, Seung Kwan

AU - Oh, Myeong Jin

AU - Paik, Dong Hyun

AU - Ryu, Tae Kyung

AU - Park, Kyeongsoon

AU - Kim, Sung Eun

AU - Park, Jong Hoon

AU - Kim, Jung Hyun

AU - Choi, Sung Wook

PY - 2013/3/12

Y1 - 2013/3/12

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AB - This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.

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A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture

Abstract

Keywords

ASJC Scopus subject areas

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