A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells

Min Hee Lee; Hoyeon Lee; Min Jung Chang; Hyeong Seok Kim; Chulhun Kang; Jong Seung Kim

doi:10.1016/j.dyepig.2016.03.032

A fluorescent probe for the Fe³⁺ ion pool in endoplasmic reticulum in liver cells

Min Hee Lee, Hoyeon Lee, Min Jung Chang, Hyeong Seok Kim, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe³⁺ ion in biological systems. In the presence of Fe³⁺ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe³⁺ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe³⁺ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

Original language	English
Pages (from-to)	245-250
Number of pages	6
Journal	Dyes and Pigments
Volume	130
DOIs	https://doi.org/10.1016/j.dyepig.2016.03.032
Publication status	Published - 2016 Jul

Keywords

Cell imaging
Fluorescent probe
Iron detection
Rhodamine

ASJC Scopus subject areas

Chemical Engineering(all)
Process Chemistry and Technology

Access to Document

10.1016/j.dyepig.2016.03.032

Cite this

@article{dac994f4daee4c4484c8ece444b48613,

title = "A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells",

abstract = "We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.",

keywords = "Cell imaging, Fluorescent probe, Iron detection, Rhodamine",

author = "Lee, {Min Hee} and Hoyeon Lee and Chang, {Min Jung} and Kim, {Hyeong Seok} and Chulhun Kang and Kim, {Jong Seung}",

note = "Funding Information: This research was supported by the NRF ( 2015R1C1A2A01054496 , MHL), the CRI project of Korea (No. 2009-0081566 , JSK) and the NRF (No. 2015R1A5A1037656 , CK). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd. All rights reserved.",

year = "2016",

month = jul,

doi = "10.1016/j.dyepig.2016.03.032",

language = "English",

volume = "130",

pages = "245--250",

journal = "Dyes and Pigments",

issn = "0143-7208",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells

AU - Lee, Min Hee

AU - Lee, Hoyeon

AU - Chang, Min Jung

AU - Kim, Hyeong Seok

AU - Kang, Chulhun

AU - Kim, Jong Seung

N1 - Funding Information: This research was supported by the NRF ( 2015R1C1A2A01054496 , MHL), the CRI project of Korea (No. 2009-0081566 , JSK) and the NRF (No. 2015R1A5A1037656 , CK). Publisher Copyright: © 2016 Elsevier Ltd. All rights reserved.

PY - 2016/7

Y1 - 2016/7

N2 - We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

AB - We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

KW - Cell imaging

KW - Fluorescent probe

KW - Iron detection

KW - Rhodamine

UR - http://www.scopus.com/inward/record.url?scp=84962273783&partnerID=8YFLogxK

U2 - 10.1016/j.dyepig.2016.03.032

DO - 10.1016/j.dyepig.2016.03.032

M3 - Article

AN - SCOPUS:84962273783

SN - 0143-7208

VL - 130

SP - 245

EP - 250

JO - Dyes and Pigments

JF - Dyes and Pigments

ER -