A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells

Min Hee Lee; Hoyeon Lee; Min Jung Chang; Hyeong Seok Kim; Chulhun Kang; Jong Seung Kim

doi:10.1016/j.dyepig.2016.03.032

A fluorescent probe for the Fe³⁺ ion pool in endoplasmic reticulum in liver cells

Min Hee Lee^*
, Hoyeon Lee
, Min Jung Chang
, Hyeong Seok Kim
, Chulhun Kang
, Jong Seung Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe³⁺ ion in biological systems. In the presence of Fe³⁺ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe³⁺ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe³⁺ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

Original language	English
Pages (from-to)	245-250
Number of pages	6
Journal	Dyes and Pigments
Volume	130
DOIs	https://doi.org/10.1016/j.dyepig.2016.03.032
Publication status	Published - 2016 Jul

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd. All rights reserved.

Keywords

Cell imaging
Fluorescent probe
Iron detection
Rhodamine

ASJC Scopus subject areas

General Chemical Engineering
Process Chemistry and Technology

Access to Document

10.1016/j.dyepig.2016.03.032

Cite this

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title = "A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells",

abstract = "We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.",

keywords = "Cell imaging, Fluorescent probe, Iron detection, Rhodamine",

author = "Lee, \{Min Hee\} and Hoyeon Lee and Chang, \{Min Jung\} and Kim, \{Hyeong Seok\} and Chulhun Kang and Kim, \{Jong Seung\}",

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T1 - A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells

AU - Lee, Min Hee

AU - Lee, Hoyeon

AU - Chang, Min Jung

AU - Kim, Hyeong Seok

AU - Kang, Chulhun

AU - Kim, Jong Seung

PY - 2016/7

Y1 - 2016/7

N2 - We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

AB - We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

KW - Cell imaging

KW - Fluorescent probe

KW - Iron detection

KW - Rhodamine

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DO - 10.1016/j.dyepig.2016.03.032

M3 - Article

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SP - 245

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