A hantavirus causing hemorrhagic fever with renal syndrome requires gC1qR/p32 for efficient cell binding and infection

Yun Choi; Young Chan Kwon; Soo In Kim; Jung Min Park; Kyung Hee Lee; Byung Yoon Ahn

doi:10.1016/j.virol.2008.08.035

A hantavirus causing hemorrhagic fever with renal syndrome requires gC1qR/p32 for efficient cell binding and infection

Yun Choi, Young Chan Kwon, Soo In Kim, Jung Min Park, Kyung Hee Lee, Byung Yoon Ahn

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Hantaan virus (HTNV) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). HTNV infection is mediated by αvβ3 integrin. We used protein blots of Vero E6 cell homogenates to demonstrate that radiolabeled HTNV virions bind to gC1qR/p32, the acidic 32-kDa protein known as the receptor for the globular head domain of complement C1q. RNAi-mediated suppression of gC1qR/p32 markedly reduced HTNV binding and infection in human lung epithelial A549 cells. Conversely, transient expression of either simian or human gC1qR/p32 rendered non-permissive CHO cells susceptible to HTNV infection. These results suggest an important role for gC1qR/p32 in HTNV infection and pathogenesis.

Original language	English
Pages (from-to)	178-183
Number of pages	6
Journal	Virology
Volume	381
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.08.035
Publication status	Published - 2008 Nov 25

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Education Foundation (R01-2002-000-00214-0 to B.Y.A.). Y.C.K. and K.H.L were supported by the BK21 Graduate Fellowship from the Ministry of Education, the Republic of Korea. We thank P. Palese (Mount Sinai) for providing NDV-GFP.

Keywords

Hantavirus
Infection
Viral binding
gC1qR/p32

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2008.08.035

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title = "A hantavirus causing hemorrhagic fever with renal syndrome requires gC1qR/p32 for efficient cell binding and infection",

abstract = "Hantaan virus (HTNV) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). HTNV infection is mediated by αvβ3 integrin. We used protein blots of Vero E6 cell homogenates to demonstrate that radiolabeled HTNV virions bind to gC1qR/p32, the acidic 32-kDa protein known as the receptor for the globular head domain of complement C1q. RNAi-mediated suppression of gC1qR/p32 markedly reduced HTNV binding and infection in human lung epithelial A549 cells. Conversely, transient expression of either simian or human gC1qR/p32 rendered non-permissive CHO cells susceptible to HTNV infection. These results suggest an important role for gC1qR/p32 in HTNV infection and pathogenesis.",

keywords = "Hantavirus, Infection, Viral binding, gC1qR/p32",

author = "Yun Choi and Kwon, {Young Chan} and Kim, {Soo In} and Park, {Jung Min} and Lee, {Kyung Hee} and Ahn, {Byung Yoon}",

note = "Funding Information: This work was supported by the Korea Science and Education Foundation (R01-2002-000-00214-0 to B.Y.A.). Y.C.K. and K.H.L were supported by the BK21 Graduate Fellowship from the Ministry of Education, the Republic of Korea. We thank P. Palese (Mount Sinai) for providing NDV-GFP.",

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doi = "10.1016/j.virol.2008.08.035",

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AU - Choi, Yun

AU - Kwon, Young Chan

AU - Kim, Soo In

AU - Park, Jung Min

AU - Lee, Kyung Hee

AU - Ahn, Byung Yoon

N1 - Funding Information: This work was supported by the Korea Science and Education Foundation (R01-2002-000-00214-0 to B.Y.A.). Y.C.K. and K.H.L were supported by the BK21 Graduate Fellowship from the Ministry of Education, the Republic of Korea. We thank P. Palese (Mount Sinai) for providing NDV-GFP.

PY - 2008/11/25

Y1 - 2008/11/25

N2 - Hantaan virus (HTNV) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). HTNV infection is mediated by αvβ3 integrin. We used protein blots of Vero E6 cell homogenates to demonstrate that radiolabeled HTNV virions bind to gC1qR/p32, the acidic 32-kDa protein known as the receptor for the globular head domain of complement C1q. RNAi-mediated suppression of gC1qR/p32 markedly reduced HTNV binding and infection in human lung epithelial A549 cells. Conversely, transient expression of either simian or human gC1qR/p32 rendered non-permissive CHO cells susceptible to HTNV infection. These results suggest an important role for gC1qR/p32 in HTNV infection and pathogenesis.

AB - Hantaan virus (HTNV) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (HFRS). HTNV infection is mediated by αvβ3 integrin. We used protein blots of Vero E6 cell homogenates to demonstrate that radiolabeled HTNV virions bind to gC1qR/p32, the acidic 32-kDa protein known as the receptor for the globular head domain of complement C1q. RNAi-mediated suppression of gC1qR/p32 markedly reduced HTNV binding and infection in human lung epithelial A549 cells. Conversely, transient expression of either simian or human gC1qR/p32 rendered non-permissive CHO cells susceptible to HTNV infection. These results suggest an important role for gC1qR/p32 in HTNV infection and pathogenesis.

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KW - Infection

KW - Viral binding

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A hantavirus causing hemorrhagic fever with renal syndrome requires gC1qR/p32 for efficient cell binding and infection

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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