A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Doyeon Kim; Sukjun Kim; Joori Park; Hee Ryung Chang; Jeeyoon Chang; Junhak Ahn; Heedo Park; Junehee Park; Narae Son; Gihyeon Kang; Jeonghun Kim; Kisoon Kim; Man Seong Park; Yoon Ki Kim; Daehyun Baek

doi:10.1038/s41467-021-25361-5

A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Doyeon Kim
, Sukjun Kim
, Joori Park
, Hee Ryung Chang
, Jeeyoon Chang
, Junhak Ahn
, Heedo Park
, Junehee Park
, Narae Son
, Gihyeon Kang
, Jeonghun Kim
, Kisoon Kim
, Man Seong Park^*
, Yoon Ki Kim^*
, Daehyun Baek^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.

Original language	English
Article number	5120
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-25361-5
Publication status	Published - 2021 Dec 1

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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title = "A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome",

abstract = "COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.",

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AU - Ahn, Junhak

AU - Park, Heedo

AU - Park, Junehee

AU - Son, Narae

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A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Abstract

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ASJC Scopus subject areas

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