A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

Ji Young Eum; Hwang Sang Youn; Youngjun Ju; Jong Min Shim; Yunxian Piao; Jinwoo Lee; Hak Sung Kim; Jungbae Kim

doi:10.1039/c3cc48044e

A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

Ji Young Eum, Hwang Sang Youn, Youngjun Ju, Jong Min Shim, Yunxian Piao, Jinwoo Lee, Hak Sung Kim, Jungbae Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.

Original language	English
Pages (from-to)	3546-3548
Number of pages	3
Journal	Chemical Communications
Volume	50
Issue number	27
DOIs	https://doi.org/10.1039/c3cc48044e
Publication status	Published - 2014 Mar 6

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Chemistry(all)
Catalysis

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abstract = "A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.",

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AU - Shim, Jong Min

AU - Piao, Yunxian

AU - Lee, Jinwoo

AU - Kim, Hak Sung

AU - Kim, Jungbae

PY - 2014/3/6

Y1 - 2014/3/6

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AB - A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.

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A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

Abstract

ASJC Scopus subject areas

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