A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris

Chang Jin Park; Yun Chul Shin; Boo Ja Lee; Ki Jeong Kim; Jeong Kook Kim; Kyung Hee Paek

doi:10.1007/s00425-005-0067-1

A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris

Chang Jin Park, Yun Chul Shin, Boo Ja Lee, Ki Jeong Kim, Jeong Kook Kim, Kyung Hee Paek^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

Abstract

Plant WRKY transcription factors were previously implicated in the alteration of gene expression in response to various pathogens. The WRKY proteins constitute a large family of plant transcription factors, whose precise functions have yet to be elucidated. Using a domain-specific differential display procedure, we isolated a WRKY gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) or Xanthomonas campestris pv . vesicatoria (Xcv). The full-length cDNA of CaWRKY-a (Capsicum annuum WRKY-a) encodes a putative polypeptide of 546 amino acids, containing two WRKY domains with a zinc finger motif. The expression of CaWRKY-a could be rapidly induced by not only chemical elicitor such as salicylic acid (SA) or ethephon but also wounding treatments. The nuclear localization of CaWRKY-a was determined in transient expression system using tobacco BY-2 cells by polyethylene glycol (PEG)-mediated transformation experiment. With oligonucleotide molecules containing the putative W-box sequences as a probe, we confirmed that CaWRKY-a protein had W-box-binding activity. These results suggest that CaWRKY-a might be involved as a transcription factor in plant defense-related signal transduction pathways.

Original language	English
Pages (from-to)	168-179
Number of pages	12
Journal	Planta
Volume	223
Issue number	2
DOIs	https://doi.org/10.1007/s00425-005-0067-1
Publication status	Published - 2006 Jan

Bibliographical note

Funding Information:
Acknowledgements We thank to the members of Dr. Paek’s laboratory for helpful discussions during the work. This study was supported partly by a grant from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center a grant (CG1223) from the Crop Functional Genomics Center funded by the Korea Ministry of Science and Technology, and BioGreen grant from the Rural Development Administration.

Keywords

HH Xanthomonas campestris
Hot pepper (Capsicum annuum L.)
Hypersensitive response
Tobacco mosaic virus
WRKY transcription factor

ASJC Scopus subject areas

Genetics
Plant Science

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10.1007/s00425-005-0067-1

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title = "A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris",

abstract = "Plant WRKY transcription factors were previously implicated in the alteration of gene expression in response to various pathogens. The WRKY proteins constitute a large family of plant transcription factors, whose precise functions have yet to be elucidated. Using a domain-specific differential display procedure, we isolated a WRKY gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) or Xanthomonas campestris pv . vesicatoria (Xcv). The full-length cDNA of CaWRKY-a (Capsicum annuum WRKY-a) encodes a putative polypeptide of 546 amino acids, containing two WRKY domains with a zinc finger motif. The expression of CaWRKY-a could be rapidly induced by not only chemical elicitor such as salicylic acid (SA) or ethephon but also wounding treatments. The nuclear localization of CaWRKY-a was determined in transient expression system using tobacco BY-2 cells by polyethylene glycol (PEG)-mediated transformation experiment. With oligonucleotide molecules containing the putative W-box sequences as a probe, we confirmed that CaWRKY-a protein had W-box-binding activity. These results suggest that CaWRKY-a might be involved as a transcription factor in plant defense-related signal transduction pathways.",

keywords = "HH Xanthomonas campestris, Hot pepper (Capsicum annuum L.), Hypersensitive response, Tobacco mosaic virus, WRKY transcription factor",

author = "Park, \{Chang Jin\} and Shin, \{Yun Chul\} and Lee, \{Boo Ja\} and Kim, \{Ki Jeong\} and Kim, \{Jeong Kook\} and Paek, \{Kyung Hee\}",

note = "Funding Information: Acknowledgements We thank to the members of Dr. Paek{\textquoteright}s laboratory for helpful discussions during the work. This study was supported partly by a grant from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center a grant (CG1223) from the Crop Functional Genomics Center funded by the Korea Ministry of Science and Technology, and BioGreen grant from the Rural Development Administration.",

year = "2006",

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doi = "10.1007/s00425-005-0067-1",

language = "English",

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AU - Park, Chang Jin

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AU - Lee, Boo Ja

AU - Kim, Ki Jeong

AU - Kim, Jeong Kook

AU - Paek, Kyung Hee

N1 - Funding Information: Acknowledgements We thank to the members of Dr. Paek’s laboratory for helpful discussions during the work. This study was supported partly by a grant from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center a grant (CG1223) from the Crop Functional Genomics Center funded by the Korea Ministry of Science and Technology, and BioGreen grant from the Rural Development Administration.

PY - 2006/1

Y1 - 2006/1

N2 - Plant WRKY transcription factors were previously implicated in the alteration of gene expression in response to various pathogens. The WRKY proteins constitute a large family of plant transcription factors, whose precise functions have yet to be elucidated. Using a domain-specific differential display procedure, we isolated a WRKY gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) or Xanthomonas campestris pv . vesicatoria (Xcv). The full-length cDNA of CaWRKY-a (Capsicum annuum WRKY-a) encodes a putative polypeptide of 546 amino acids, containing two WRKY domains with a zinc finger motif. The expression of CaWRKY-a could be rapidly induced by not only chemical elicitor such as salicylic acid (SA) or ethephon but also wounding treatments. The nuclear localization of CaWRKY-a was determined in transient expression system using tobacco BY-2 cells by polyethylene glycol (PEG)-mediated transformation experiment. With oligonucleotide molecules containing the putative W-box sequences as a probe, we confirmed that CaWRKY-a protein had W-box-binding activity. These results suggest that CaWRKY-a might be involved as a transcription factor in plant defense-related signal transduction pathways.

AB - Plant WRKY transcription factors were previously implicated in the alteration of gene expression in response to various pathogens. The WRKY proteins constitute a large family of plant transcription factors, whose precise functions have yet to be elucidated. Using a domain-specific differential display procedure, we isolated a WRKY gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) or Xanthomonas campestris pv . vesicatoria (Xcv). The full-length cDNA of CaWRKY-a (Capsicum annuum WRKY-a) encodes a putative polypeptide of 546 amino acids, containing two WRKY domains with a zinc finger motif. The expression of CaWRKY-a could be rapidly induced by not only chemical elicitor such as salicylic acid (SA) or ethephon but also wounding treatments. The nuclear localization of CaWRKY-a was determined in transient expression system using tobacco BY-2 cells by polyethylene glycol (PEG)-mediated transformation experiment. With oligonucleotide molecules containing the putative W-box sequences as a probe, we confirmed that CaWRKY-a protein had W-box-binding activity. These results suggest that CaWRKY-a might be involved as a transcription factor in plant defense-related signal transduction pathways.

KW - HH Xanthomonas campestris

KW - Hot pepper (Capsicum annuum L.)

KW - Hypersensitive response

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KW - WRKY transcription factor

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IS - 2

ER -

A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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