A low fluence Q-switched Nd:YAG laser modifies the 3D structure of melanocyte and ultrastructure of melanosome by subcellular-selective photothermolysis

Ji Young Mun, Se Yeong Jeong, Jae Hwan Kim, Sung Sik Han, Il Hwan Kim

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    111 Citations (Scopus)

    Abstract

    Laser treatment using low fluence for melasma was previously introduced to overcome postinflammatory hypermelanosis after Q-switched laser therapy. However, research on the mechanism of this treatment is very limited. In this study, a collimated low fluence 1064 nm Q-switched Nd:YAG laser with a pulse width of <7 ns was applied using top-hat beam mode. The aim of this study was to investigate the mode of action of this laser treatment through electron microscopy. The effectiveness of this treatment was confirmed by clinical photos, melasma area and severity index and spectrophotometer. To understand the mode of action, the three-dimensional structure of melanocytes in the epidermis was analyzed using serial images acquired by a 3VIEW surface block face scanning electron microscope. In the epidermis, after laser treatment, fewer dendrites in the melanocytes were observed compared with pretreatment. In addition, ultrastructural changes in the melanosome were studied using transmission electron microscopy, which showed that laser treatment caused selective photothermolysis on Stage IV melanosome. Therefore, this treatment should be regarded as an effective method for treating melasma through subcellular-selective photothermolysis.

    Original languageEnglish
    Pages (from-to)11-18
    Number of pages8
    JournalJournal of Electron Microscopy
    Volume60
    Issue number1
    DOIs
    Publication statusPublished - 2011 Feb

    Bibliographical note

    Funding Information:
    This work was supported by Lutronic corporation and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (KRF-2008-314-C00278).

    Keywords

    • 3D structure
    • 3VIEW SBF-SEM
    • laser treatment
    • melanocyte
    • melasma
    • melnosome

    ASJC Scopus subject areas

    • Instrumentation

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