A metabolomic approach to determine the geographical origins of Anemarrhena asphodeloides by using UPLC-QTOF MS

Nahyun Kim, Seung Mok Ryu, Dong Hyuk Lee, Jae Won Lee, Eun Kyoung Seo, Je Hyun Lee, Dongho Lee

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


An ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF MS) method was developed for metabolite profiling of Anemarrhena asphodeloides Bunge from two different geographical origins. In this study, the metabolite profile data obtained using UPLC-QTOF MS was subjected to multivariate statistical analyses, such as the principal component analysis and the hierarchical clustering analysis, to compare metabolite patterns among A. asphodeloides samples. Furthermore, a metabolite selection method known as significance analysis of microarrays (SAM) was applied to further select metabolites and to identify key constituents to efficiently distinguish between geographical origins. The UPLC-QTOF MS analysis successfully classified 21 samples into two distinct groups according to their geographical origins. The validation method used to assess the analytical stability and accuracy of these data is also described. These results suggest that this proposed method is reliable, accurate, and effective for geographic classification of A. asphodeloides, thus guiding its proper use for therapeutic purposes.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - 2014 Apr 15

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Food and Drug Administration for Studies on Standardization of Herbal Medicine (2008) and a Korea University Grant .


  • Anemarrhena asphodeloides
  • Metabolite profiling
  • Metabolite selection
  • Method validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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