A micellar complex of a conjugated polyelectrolyte for efficient FRET to dye-labeled DNA

Rati Ranjan Nayak; Okhil Kumar Nag; Mijeong Kang; Youngeup Jin; Hongsuk Suh; Kwanghee Lee; Han Young Woo

doi:10.1002/marc.200800763

A micellar complex of a conjugated polyelectrolyte for efficient FRET to dye-labeled DNA

Rati Ranjan Nayak, Okhil Kumar Nag, Mijeong Kang, Youngeup Jin, Hongsuk Suh, Kwanghee Lee, Han Young Woo

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.

Original language	English
Pages (from-to)	633-638
Number of pages	6
Journal	Macromolecular Rapid Communications
Volume	30
Issue number	8
DOIs	https://doi.org/10.1002/marc.200800763
Publication status	Published - 2009 Apr 20
Externally published	Yes

Keywords

Conjugated polyelectrolytes
Donor-acceptor systems
FRET
Fluorescence
Surfactants

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1002/marc.200800763

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title = "A micellar complex of a conjugated polyelectrolyte for efficient FRET to dye-labeled DNA",

abstract = "A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.",

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author = "Nayak, {Rati Ranjan} and Nag, {Okhil Kumar} and Mijeong Kang and Youngeup Jin and Hongsuk Suh and Kwanghee Lee and Woo, {Han Young}",

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T1 - A micellar complex of a conjugated polyelectrolyte for efficient FRET to dye-labeled DNA

AU - Nayak, Rati Ranjan

AU - Nag, Okhil Kumar

AU - Kang, Mijeong

AU - Jin, Youngeup

AU - Suh, Hongsuk

AU - Lee, Kwanghee

AU - Woo, Han Young

PY - 2009/4/20

Y1 - 2009/4/20

N2 - A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.

AB - A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in the presence of non-ionic surfactants. A TEM microscopic study shows the formation of a nanowire micellar complex of c-PFP and the surfactants. About a 400% enhancement of the FRET signal is measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification is successfully modulated using different types of non-ionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation), and the resulting energy transfer process.

KW - Conjugated polyelectrolytes

KW - Donor-acceptor systems

KW - FRET

KW - Fluorescence

KW - Surfactants

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SN - 1022-1336

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IS - 8

ER -

A micellar complex of a conjugated polyelectrolyte for efficient FRET to dye-labeled DNA

Abstract

Keywords

ASJC Scopus subject areas

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