A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes

Shaopei Li; Yangzhi Zhu; Reihaneh Haghniaz; Satoru Kawakita; Shenghan Guan; Jianjun Chen; Zijie Li; Kalpana Mandal; Jamal Bahari; Shilp Shah; Juchen Guo; Heemin Kang; Wujin Sun; Han Jun Kim; Vadim Jucaud; Mehmet R. Dokmeci; Pete Kollbaum; Chi Hwan Lee; Ali Khademhosseini

doi:10.1002/adfm.202206620

A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes

Shaopei Li, Yangzhi Zhu, Reihaneh Haghniaz, Satoru Kawakita, Shenghan Guan, Jianjun Chen, Zijie Li, Kalpana Mandal, Jamal Bahari, Shilp Shah, Juchen Guo, Heemin Kang, Wujin Sun, Han Jun Kim, Vadim Jucaud, Mehmet R. Dokmeci, Pete Kollbaum, Chi Hwan Lee, Ali Khademhosseini

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Exosomes, a form of small extracellular vesicles, play a crucial role in the metastasis of cancers and thus are investigated as potential biomarkers for cancer diagnosis. However, conventional detection methods like immune-based assay and microRNA analyses are expensive and require tedious pretreatments and lengthy analysis time. Since exosomes related to cancers are reported to exist in tears, a poly(2-hydroxyethyl methacrylate) contact lens embedded with antibody-conjugated signaling microchambers (ACSM-PCL) capable of detecting tear exosomes is reported. The ACSM-PCL exhibits high optical transparency and mechanical properties, along with extraordinary biocompatibility and good sensitivity to exosomes. A gold nanoparticle colorimetric assay is employed to visualize captured exosomes. The ACSM-PCL can detect exosomes in the pH range of 6.5–7.4 (similar to the human tear pH) and have a strong recovery yield in bovine serum albumin solutions. In particular, the ACSM-PCL can detect exosomes in various solutions, including regular buffer, cell culture media from various cell lines, and human tears. Finally, the ACSM-PCL can differentiate expression of exosome surface proteins hypothesized as cancer biomarkers. With these encouraging results, this ACSM-PCL is promised to be the next generation smart contact lens as an easy-to-use, rapid, noninvasive monitoring platform of cancer pre-screening and supportive diagnosis.

Original language	English
Article number	2206620
Journal	Advanced Functional Materials
Volume	32
Issue number	44
DOIs	https://doi.org/10.1002/adfm.202206620
Publication status	Published - 2022 Oct 26

Keywords

biomarkers
biosensors
cancer diagnosis
contact lenses
exosomes
wearables

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Chemistry(all)
Materials Science(all)
Electrochemistry
Biomaterials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/adfm.202206620

Cite this

Li, S., Zhu, Y., Haghniaz, R., Kawakita, S., Guan, S., Chen, J., Li, Z., Mandal, K., Bahari, J., Shah, S., Guo, J., Kang, H., Sun, W., Kim, H. J., Jucaud, V., Dokmeci, M. R., Kollbaum, P., Lee, C. H., & Khademhosseini, A. (2022). A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes. Advanced Functional Materials, 32(44), Article 2206620. https://doi.org/10.1002/adfm.202206620

Li, S, Zhu, Y, Haghniaz, R, Kawakita, S, Guan, S, Chen, J, Li, Z, Mandal, K, Bahari, J, Shah, S, Guo, J, Kang, H, Sun, W, Kim, HJ, Jucaud, V, Dokmeci, MR, Kollbaum, P, Lee, CH & Khademhosseini, A 2022, 'A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes', Advanced Functional Materials, vol. 32, no. 44, 2206620. https://doi.org/10.1002/adfm.202206620

@article{93f46498c22845ca95618bb6a6545e73,

title = "A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes",

abstract = "Exosomes, a form of small extracellular vesicles, play a crucial role in the metastasis of cancers and thus are investigated as potential biomarkers for cancer diagnosis. However, conventional detection methods like immune-based assay and microRNA analyses are expensive and require tedious pretreatments and lengthy analysis time. Since exosomes related to cancers are reported to exist in tears, a poly(2-hydroxyethyl methacrylate) contact lens embedded with antibody-conjugated signaling microchambers (ACSM-PCL) capable of detecting tear exosomes is reported. The ACSM-PCL exhibits high optical transparency and mechanical properties, along with extraordinary biocompatibility and good sensitivity to exosomes. A gold nanoparticle colorimetric assay is employed to visualize captured exosomes. The ACSM-PCL can detect exosomes in the pH range of 6.5–7.4 (similar to the human tear pH) and have a strong recovery yield in bovine serum albumin solutions. In particular, the ACSM-PCL can detect exosomes in various solutions, including regular buffer, cell culture media from various cell lines, and human tears. Finally, the ACSM-PCL can differentiate expression of exosome surface proteins hypothesized as cancer biomarkers. With these encouraging results, this ACSM-PCL is promised to be the next generation smart contact lens as an easy-to-use, rapid, noninvasive monitoring platform of cancer pre-screening and supportive diagnosis.",

keywords = "biomarkers, biosensors, cancer diagnosis, contact lenses, exosomes, wearables",

author = "Shaopei Li and Yangzhi Zhu and Reihaneh Haghniaz and Satoru Kawakita and Shenghan Guan and Jianjun Chen and Zijie Li and Kalpana Mandal and Jamal Bahari and Shilp Shah and Juchen Guo and Heemin Kang and Wujin Sun and Kim, {Han Jun} and Vadim Jucaud and Dokmeci, {Mehmet R.} and Pete Kollbaum and Lee, {Chi Hwan} and Ali Khademhosseini",

note = "Funding Information: The authors acknowledge the colleagues from the Terasaki Institute for Biomedical Innovation, the University of California, San Diego, and the University of California, Riverside, for helpful discussions. Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",

year = "2022",

month = oct,

day = "26",

doi = "10.1002/adfm.202206620",

language = "English",

volume = "32",

journal = "Advanced Functional Materials",

issn = "1616-301X",

publisher = "Wiley-VCH Verlag",

number = "44",

}

TY - JOUR

T1 - A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes

AU - Li, Shaopei

AU - Zhu, Yangzhi

AU - Haghniaz, Reihaneh

AU - Kawakita, Satoru

AU - Guan, Shenghan

AU - Chen, Jianjun

AU - Li, Zijie

AU - Mandal, Kalpana

AU - Bahari, Jamal

AU - Shah, Shilp

AU - Guo, Juchen

AU - Kang, Heemin

AU - Sun, Wujin

AU - Kim, Han Jun

AU - Jucaud, Vadim

AU - Dokmeci, Mehmet R.

AU - Kollbaum, Pete

AU - Lee, Chi Hwan

AU - Khademhosseini, Ali

N1 - Funding Information: The authors acknowledge the colleagues from the Terasaki Institute for Biomedical Innovation, the University of California, San Diego, and the University of California, Riverside, for helpful discussions. Publisher Copyright: © 2022 Wiley-VCH GmbH.

PY - 2022/10/26

Y1 - 2022/10/26

N2 - Exosomes, a form of small extracellular vesicles, play a crucial role in the metastasis of cancers and thus are investigated as potential biomarkers for cancer diagnosis. However, conventional detection methods like immune-based assay and microRNA analyses are expensive and require tedious pretreatments and lengthy analysis time. Since exosomes related to cancers are reported to exist in tears, a poly(2-hydroxyethyl methacrylate) contact lens embedded with antibody-conjugated signaling microchambers (ACSM-PCL) capable of detecting tear exosomes is reported. The ACSM-PCL exhibits high optical transparency and mechanical properties, along with extraordinary biocompatibility and good sensitivity to exosomes. A gold nanoparticle colorimetric assay is employed to visualize captured exosomes. The ACSM-PCL can detect exosomes in the pH range of 6.5–7.4 (similar to the human tear pH) and have a strong recovery yield in bovine serum albumin solutions. In particular, the ACSM-PCL can detect exosomes in various solutions, including regular buffer, cell culture media from various cell lines, and human tears. Finally, the ACSM-PCL can differentiate expression of exosome surface proteins hypothesized as cancer biomarkers. With these encouraging results, this ACSM-PCL is promised to be the next generation smart contact lens as an easy-to-use, rapid, noninvasive monitoring platform of cancer pre-screening and supportive diagnosis.

AB - Exosomes, a form of small extracellular vesicles, play a crucial role in the metastasis of cancers and thus are investigated as potential biomarkers for cancer diagnosis. However, conventional detection methods like immune-based assay and microRNA analyses are expensive and require tedious pretreatments and lengthy analysis time. Since exosomes related to cancers are reported to exist in tears, a poly(2-hydroxyethyl methacrylate) contact lens embedded with antibody-conjugated signaling microchambers (ACSM-PCL) capable of detecting tear exosomes is reported. The ACSM-PCL exhibits high optical transparency and mechanical properties, along with extraordinary biocompatibility and good sensitivity to exosomes. A gold nanoparticle colorimetric assay is employed to visualize captured exosomes. The ACSM-PCL can detect exosomes in the pH range of 6.5–7.4 (similar to the human tear pH) and have a strong recovery yield in bovine serum albumin solutions. In particular, the ACSM-PCL can detect exosomes in various solutions, including regular buffer, cell culture media from various cell lines, and human tears. Finally, the ACSM-PCL can differentiate expression of exosome surface proteins hypothesized as cancer biomarkers. With these encouraging results, this ACSM-PCL is promised to be the next generation smart contact lens as an easy-to-use, rapid, noninvasive monitoring platform of cancer pre-screening and supportive diagnosis.

KW - biomarkers

KW - biosensors

KW - cancer diagnosis

KW - contact lenses

KW - exosomes

KW - wearables

UR - http://www.scopus.com/inward/record.url?scp=85135620960&partnerID=8YFLogxK

U2 - 10.1002/adfm.202206620

DO - 10.1002/adfm.202206620

M3 - Article

AN - SCOPUS:85135620960

SN - 1616-301X

VL - 32

JO - Advanced Functional Materials

JF - Advanced Functional Materials

IS - 44

M1 - 2206620

ER -

A Microchambers Containing Contact Lens for the Noninvasive Detection of Tear Exosomes

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this