A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions

Jungil Park, Youngmi Kim Pak, James Jungho Pak

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate (OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode (WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The fabricated sensor showed a sensitivity of 2.84 A/cm2 M and a 90% response time of 4.9 s in an average of 5 sensors when a 25,000 μm2 WE and a 20 μm polydimethylsiloxane membrane were used. This is the first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05 ± 1.87 nA at the full-oxygen state and 2.77 ± 1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69 ± 0.30 was almost identical to the result of 3.70 ± 0.26 obtained from a commercial system.

    Original languageEnglish
    Pages (from-to)263-269
    Number of pages7
    JournalSensors and Actuators, B: Chemical
    Volume147
    Issue number1
    DOIs
    Publication statusPublished - 2010 May 18

    Keywords

    • Cellular respiration
    • Electrochemistry
    • Oxygen consumption rate
    • Oxygen sensor
    • Reservoir-type

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Instrumentation
    • Condensed Matter Physics
    • Surfaces, Coatings and Films
    • Metals and Alloys
    • Electrical and Electronic Engineering
    • Materials Chemistry

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