A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Kisuk Yang; Sewoon Han; Yoojin Shin; Eunkyung Ko; Jin Kim; Kook In Park; Seok Chung; Seung Woo Cho

doi:10.1016/j.biomaterials.2013.05.067

A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Kisuk Yang, Sewoon Han, Yoojin Shin, Eunkyung Ko, Jin Kim, Kook In Park, Seok Chung, Seung Woo Cho

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.

Original language	English
Pages (from-to)	6607-6614
Number of pages	8
Journal	Biomaterials
Volume	34
Issue number	28
DOIs	https://doi.org/10.1016/j.biomaterials.2013.05.067
Publication status	Published - 2013 Sept

Bibliographical note

Funding Information:
This work was supported by grants (S.W. Cho, 2010-0020409 and 2010-0022037 ) funded by the National Research Foundation of Korea, the Ministry of Education, Science and Technology, Republic of Korea . We also acknowledge supports to S. Chung from the Ministry for Health & Welfare Affairs ( A092255 ) and the National Research Foundation of Korea ( NRF-2012M3A6A-2011-0032211 ).

Keywords

Differentiation
Hypoxia
Microfluidic array
Neural stem cells
Self-renewal

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2013.05.067

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title = "A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment",

abstract = "We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.",

keywords = "Differentiation, Hypoxia, Microfluidic array, Neural stem cells, Self-renewal",

author = "Kisuk Yang and Sewoon Han and Yoojin Shin and Eunkyung Ko and Jin Kim and Park, {Kook In} and Seok Chung and Cho, {Seung Woo}",

note = "Funding Information: This work was supported by grants (S.W. Cho, 2010-0020409 and 2010-0022037 ) funded by the National Research Foundation of Korea, the Ministry of Education, Science and Technology, Republic of Korea . We also acknowledge supports to S. Chung from the Ministry for Health & Welfare Affairs ( A092255 ) and the National Research Foundation of Korea ( NRF-2012M3A6A-2011-0032211 ).",

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doi = "10.1016/j.biomaterials.2013.05.067",

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T1 - A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

AU - Yang, Kisuk

AU - Han, Sewoon

AU - Shin, Yoojin

AU - Ko, Eunkyung

AU - Kim, Jin

AU - Park, Kook In

AU - Chung, Seok

AU - Cho, Seung Woo

N1 - Funding Information: This work was supported by grants (S.W. Cho, 2010-0020409 and 2010-0022037 ) funded by the National Research Foundation of Korea, the Ministry of Education, Science and Technology, Republic of Korea . We also acknowledge supports to S. Chung from the Ministry for Health & Welfare Affairs ( A092255 ) and the National Research Foundation of Korea ( NRF-2012M3A6A-2011-0032211 ).

PY - 2013/9

Y1 - 2013/9

N2 - We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.

AB - We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an invivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.

KW - Differentiation

KW - Hypoxia

KW - Microfluidic array

KW - Neural stem cells

KW - Self-renewal

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A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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