We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Ministry of Science and ICT (MSIT) (Grant number 2019R1A2C2008863, 2019M3A9H2032547, 2016R1A6A1A03012845).
© 2020 The Royal Society of Chemistry.
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry