A mitochondrial-targeted two-photon probe for zinc ion

Goutam Masanta; Chang Su Lim; Hyung Joong Kim; Ji Hee Han; Hwan Myung Kim; Bong Rae Cho

doi:10.1021/ja200444t

A mitochondrial-targeted two-photon probe for zinc ion

Goutam Masanta
, Chang Su Lim
, Hyung Joong Kim
, Ji Hee Han
, Hwan Myung Kim
, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

237 Citations (Scopus)

Abstract

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn²⁺]_m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn²⁺ with a dissociation constant (KdTP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn²⁺] _m in a rat hippocampal slice at a depth of 100-200 μm without interference from other metal ions through the use of two-photon microscopy.

Original language	English
Pages (from-to)	5698-5700
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	15
DOIs	https://doi.org/10.1021/ja200444t
Publication status	Published - 2011 Apr 20

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja200444t

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title = "A mitochondrial-targeted two-photon probe for zinc ion",

abstract = "We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (KdTP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+] m in a rat hippocampal slice at a depth of 100-200 μm without interference from other metal ions through the use of two-photon microscopy.",

author = "Goutam Masanta and Lim, \{Chang Su\} and Kim, \{Hyung Joong\} and Han, \{Ji Hee\} and Kim, \{Hwan Myung\} and Cho, \{Bong Rae\}",

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AU - Masanta, Goutam

AU - Lim, Chang Su

AU - Kim, Hyung Joong

AU - Han, Ji Hee

AU - Kim, Hwan Myung

AU - Cho, Bong Rae

PY - 2011/4/20

Y1 - 2011/4/20

N2 - We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (KdTP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+] m in a rat hippocampal slice at a depth of 100-200 μm without interference from other metal ions through the use of two-photon microscopy.

AB - We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (KdTP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+] m in a rat hippocampal slice at a depth of 100-200 μm without interference from other metal ions through the use of two-photon microscopy.

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UR - https://www.scopus.com/pages/publications/79954567773#tab=citedBy

U2 - 10.1021/ja200444t

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EP - 5700

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 15

ER -

A mitochondrial-targeted two-photon probe for zinc ion

Abstract

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