A molecular marker associated with the H21 Hessian fly resistance gene in wheat

Y. W. Seo, J. W. Johnson, R. L. Jarret

    Research output: Contribution to journalArticlepeer-review

    38 Citations (Scopus)

    Abstract

    Near-isogenic lines in conjunction with bulked segregant analysis were used to identify a DNA marker in wheat (Triticum aestivum L.) associated with the H21 gene conferring resistance to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. Near-isogenic lines were developed by backcross introgression BC3F3:4 ('Coker 797' * 4 / 'Hamlet') and differed by the presence or absence of H21 (on 2RL) derived from 'Chaupon' rye (Secale cereale L.). Bulked DNA samples were prepared from near-isogenic lines and BC3F2 population individuals segregating for reaction to Hessian fly biotype L and screened for random amplified polymorphic DNA markers using 46 10mer primers. Random-amplified polymorphic DNA markers from resistant and susceptible individuals and parental lines were scored and these data were used to identify a 3 kb DNA fragment that was related to the occurrence of H21. This fragment was amplified from DNA isolated from Hamlet, a near-isogenic line carrying 2RL, and bulked DNA from resistant BC3F2 individuals, but not from the recurrent parent Coker 797 or DNA bulks from susceptible BC3F2 plants. Analysis of 111 BC3F2 segregating individuals and BC3F2:3 segregants confirmed the co-segregation of the 3 kb DNA marker with the H21 resistance gene to Hessian fly. Use of this marker could facilitate more rapid screening of plant populations for Hessian fly resistance and monitoring the introgression of H21.

    Original languageEnglish
    Pages (from-to)177-181
    Number of pages5
    JournalMolecular Breeding
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - 1997

    Keywords

    • Bulked segregant analysis
    • H21
    • Near-isogenic lines (NILs)
    • RAPD
    • Wheat

    ASJC Scopus subject areas

    • Biotechnology
    • Molecular Biology
    • Agronomy and Crop Science
    • Genetics
    • Plant Science

    Fingerprint

    Dive into the research topics of 'A molecular marker associated with the H21 Hessian fly resistance gene in wheat'. Together they form a unique fingerprint.

    Cite this