A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Seyoung Lim; Jung Woong Choi; Hyeon Soo Kim; Yun Hee Kim; Kyungmoo Yea; Kyun Heo; Jong Hyun Kim; Sun Hee Kim; Minseok Song; Jae Il Kim; Sung Ho Ryu; Pann Ghill Suh

doi:10.1016/j.lfs.2008.01.005

A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Seyoung Lim, Jung Woong Choi, Hyeon Soo Kim, Yun Hee Kim, Kyungmoo Yea, Kyun Heo, Jong Hyun Kim, Sun Hee Kim, Minseok Song, Jae Il Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca⁺²]_i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca⁺²]_i generation was completely abrogated by phospholipase C (PLC), IP₃ receptor or Gα_i/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gα_i/o, PLC and IP₃-dependent pathways.

Original language	English
Pages (from-to)	733-740
Number of pages	8
Journal	Life Sciences
Volume	82
Issue number	13-14
DOIs	https://doi.org/10.1016/j.lfs.2008.01.005
Publication status	Published - 2008 Mar 26
Externally published	Yes

Keywords

Calcium
Degranulation
IP
Mast cells
Phospholipase C
ζ-PS

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/j.lfs.2008.01.005

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@article{e61bf3a766504c01b3a84fb742bd9e82,

title = "A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity",

abstract = "Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.",

keywords = "Calcium, Degranulation, IP, Mast cells, Phospholipase C, ζ-PS",

author = "Seyoung Lim and Choi, {Jung Woong} and Kim, {Hyeon Soo} and Kim, {Yun Hee} and Kyungmoo Yea and Kyun Heo and Kim, {Jong Hyun} and Kim, {Sun Hee} and Minseok Song and Kim, {Jae Il} and Ryu, {Sung Ho} and Suh, {Pann Ghill}",

note = "Funding Information: This work was supported by the Next Generation New Technology Development Program (10027891) funded by Ministry of Commerce, Industry and Energy (MOCIE) of South Korea.",

year = "2008",

month = mar,

day = "26",

doi = "10.1016/j.lfs.2008.01.005",

language = "English",

volume = "82",

pages = "733--740",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "13-14",

}

TY - JOUR

T1 - A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

AU - Lim, Seyoung

AU - Choi, Jung Woong

AU - Kim, Hyeon Soo

AU - Kim, Yun Hee

AU - Yea, Kyungmoo

AU - Heo, Kyun

AU - Kim, Jong Hyun

AU - Kim, Sun Hee

AU - Song, Minseok

AU - Kim, Jae Il

AU - Ryu, Sung Ho

AU - Suh, Pann Ghill

N1 - Funding Information: This work was supported by the Next Generation New Technology Development Program (10027891) funded by Ministry of Commerce, Industry and Energy (MOCIE) of South Korea.

PY - 2008/3/26

Y1 - 2008/3/26

N2 - Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.

AB - Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.

KW - Calcium

KW - Degranulation

KW - IP

KW - Mast cells

KW - Phospholipase C

KW - ζ-PS

UR - http://www.scopus.com/inward/record.url?scp=40849094196&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2008.01.005

DO - 10.1016/j.lfs.2008.01.005

M3 - Article

C2 - 18289606

AN - SCOPUS:40849094196

SN - 0024-3205

VL - 82

SP - 733

EP - 740

JO - Life Sciences

JF - Life Sciences

IS - 13-14

ER -

A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Abstract

Keywords

ASJC Scopus subject areas

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