A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Seyoung Lim, Jung Woong Choi, Hyeon Soo Kim, Yun Hee Kim, Kyungmoo Yea, Kyun Heo, Jong Hyun Kim, Sun Hee Kim, Minseok Song, Jae Il Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways.

Original languageEnglish
Pages (from-to)733-740
Number of pages8
JournalLife Sciences
Volume82
Issue number13-14
DOIs
Publication statusPublished - 2008 Mar 26
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Next Generation New Technology Development Program (10027891) funded by Ministry of Commerce, Industry and Energy (MOCIE) of South Korea.

Keywords

  • Calcium
  • Degranulation
  • IP
  • Mast cells
  • Phospholipase C
  • ζ-PS

ASJC Scopus subject areas

  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry,Genetics and Molecular Biology

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