A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

Arfaxad Reyes-Alcaraz; Emilio Y. Lucero Garcia-Rojas; Elizabeth A. Merlinsky; Jae Young Seong; Richard A. Bond; Bradley K. McConnell

doi:10.1038/s42003-022-03163-9

A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

Arfaxad Reyes-Alcaraz, Emilio Y. Lucero Garcia-Rojas, Elizabeth A. Merlinsky, Jae Young Seong, Richard A. Bond, Bradley K. McConnell

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.

Original language	English
Article number	212
Journal	Communications Biology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/s42003-022-03163-9
Publication status	Published - 2022 Dec

Bibliographical note

Publisher Copyright:
© 2022, The Author(s).

ASJC Scopus subject areas

Medicine (miscellaneous)
General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s42003-022-03163-9

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abstract = "Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.",

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AU - Reyes-Alcaraz, Arfaxad

AU - Lucero Garcia-Rojas, Emilio Y.

AU - Merlinsky, Elizabeth A.

AU - Seong, Jae Young

AU - Bond, Richard A.

AU - McConnell, Bradley K.

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AB - Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.

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A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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