A nanoplasmonic biosensor for label-free multiplex detection of cancer biomarkers

Jong Uk Lee, Anh H. Nguyen, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)


The development of highly sensitive, selective and multiplex sensors has become an important challenge for disease diagnosis. In this study, we describe a multiplex biosensor for the detection of cancer biomarkers based on unique plasmon response of single gold nanoparticles (AuNPs) and antibody-antigen binding activity. To demonstrate the ability of the plasmon biosensor to detect and quantify cancer biomarkers: a panel of biomarkers, including α-fetoprotein (AFP), carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) was used as a model analyte for multiple detection. A novel and sensitive multiplex biosensor was developed by immobilizing plasmonic nanoparticles in a site-specific manner and functionalized with monoclonal antibodies that recognize the target protein on hydrophilic-hydrophobic patterned glass slide. The proposed multi-analyte biosensor exhibited outstanding selectivity and sensitivity. The limit of detection was determined to be 91. fM, 94. fM and 10. fM for AFP, CEA and PSA from patient-mimicked serum, respectively. Finally, using this sensing strategy, this platform presents an excellent approach for versatile molecular diagnostics in both research and clinical medical fields.

Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalBiosensors and Bioelectronics
Publication statusPublished - 2015 Dec 5


  • Cancer biomarkers
  • Gold nanoparticles
  • Localized surface plasmon resonance
  • Multiplex detection

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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