A neural probe for concurrent real-time measurement of multiple neurochemicals with electrophysiology in multiple brain regions in vivo

Uikyu Chae, Jiwan Woo, Yakdol Cho, Jeong Kyu Han, Soo Hyun Yang, Esther Yang, Hyogeun Shin, Hyun Kim, Hyun Yong Yu, C. Justin Lee, Il Joo Cho

Research output: Contribution to journalArticlepeer-review

Abstract

Real-time monitoring of various neurochemicals with high spatial resolution in multiple brain regions in vivo can elucidate neural circuits related to various brain diseases. However, previous systems for monitoring neurochemicals have limitations in observing multiple neurochemicals without crosstalk in real time, and these methods cannot record electrical activity, which is essential for investigating neural circuits. Here, we present a real-time bimodal (RTBM) neural probe that uses monolithically integrated biosensors and multiple shanks to study the connectivity of neural circuits by measuring multiple neurochemicals and electrical neural activity in real time. Using the RTBM probe, we demonstrate concurrent measurements of four neurochemicals—glucose, lactate, choline, and glutamate without cross-talking each other—and electrical activity in real time in vivo. Additionally, we show the functional connectivity between the medial prefrontal cortex and mediodorsal thalamus through the simultaneous measurement of chemical and electrical signals. We expect that our device will contribute to not only elucidating the role of neurochemicals in neural circuits related to brain functions but also developing drugs for various brain diseases related to neurochemicals.

Original languageEnglish
Article numbere2219231120
JournalProceedings of the National Academy of Sciences of the United States of America
Volume120
Issue number28
DOIs
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
Copyright © 2023 the Author(s).

Keywords

  • MEMS
  • biosensor
  • neural circuit
  • neural probe
  • neurotransmitter

ASJC Scopus subject areas

  • General

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