A new α-pyrone analog, arthrifuranone A (1) was isolated from an EtOAc-extract of Arthrinium pseudosinense culture medium. The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The chemical structure of the new compound was elucidated using MS and NMR spectroscopic techniques, and the absolute configuration was established by the Mosher’s method and gauge-including atomic orbital NMR chemical shift calculations, followed by DP4 + analysis. The isolated compound was evaluated for its estrogenic activity using the MCF-7 estrogen responsive human breast cancer cells. Compound 1 showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 3.125 μM via phosphorylation of estrogen receptor-α.
Bibliographical noteFunding Information:
Acknowledgements This research was funded by Korea University, Korea Polar Research Institute (Grant: PE21150), and the National Research Foundation of Korea (NRF-2019R1A2C1006226 and NRF-2019R1A4A1020626).
© 2021, The Author(s), under exclusive licence to the Japan Antibiotics Research Association.
ASJC Scopus subject areas
- Drug Discovery