TY - CHAP
T1 - A new and simple transection knife for study of neurodegeneration and neuroregeneration in animal model
AU - Park, Jung Yul
AU - Kim, Sang-Dae
AU - Park, J.
AU - Lim, Dong Jun
AU - Lee, H. K.
AU - Chung, H. S.
PY - 2006/1/1
Y1 - 2006/1/1
N2 - The purpose of this study was to design and make a simple, inexpensive brain knife that could produce consistent results following transection in animal model. After testing various materials including commercially available products, microelectrode recording needles as used in deep brain stimulation (DBS) surgery were selected as ideal candidates. They were modified to serve as type of wire-knife for the purposes of study. For this study, the major pathway for dopaminergic neuron from substantia nigra to striatum was selected for transection. A total of 40 Sprague-Dawley rats were assigned to 8 groups; normal, 1-4, 6, 8, and 10 weeks post-transection. Degree of cell death was determined and surviving neurons were counted by means of fluorescent microscopic examination, immunohistochemistry involving tyrosine hydroxylase (TH)- immunoreactive staining, and mapping to verify complete transection. Compared to control, percentage of remaining neurons in each group was 61.3, 36.8, 29.9, 5.1, 5.9, 7.0%, respectively. Completeness of lesion was correlated with the absence of TH-immunoreactivity in the striatum. Our model seems to provide complete cell death in early period after transection with consistent results. Thus, this type of brain knife can be very handy, without any extra cost, in any research model involving transection of fiber bundle for studies on neurodegeneration and neuroregeneration.
AB - The purpose of this study was to design and make a simple, inexpensive brain knife that could produce consistent results following transection in animal model. After testing various materials including commercially available products, microelectrode recording needles as used in deep brain stimulation (DBS) surgery were selected as ideal candidates. They were modified to serve as type of wire-knife for the purposes of study. For this study, the major pathway for dopaminergic neuron from substantia nigra to striatum was selected for transection. A total of 40 Sprague-Dawley rats were assigned to 8 groups; normal, 1-4, 6, 8, and 10 weeks post-transection. Degree of cell death was determined and surviving neurons were counted by means of fluorescent microscopic examination, immunohistochemistry involving tyrosine hydroxylase (TH)- immunoreactive staining, and mapping to verify complete transection. Compared to control, percentage of remaining neurons in each group was 61.3, 36.8, 29.9, 5.1, 5.9, 7.0%, respectively. Completeness of lesion was correlated with the absence of TH-immunoreactivity in the striatum. Our model seems to provide complete cell death in early period after transection with consistent results. Thus, this type of brain knife can be very handy, without any extra cost, in any research model involving transection of fiber bundle for studies on neurodegeneration and neuroregeneration.
KW - cell death
KW - degeneration
KW - electrode
KW - Knife
KW - substantia nigra
KW - transection
UR - http://www.scopus.com/inward/record.url?scp=34248371254&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248371254&partnerID=8YFLogxK
U2 - 10.1007/978-3-211-35205-2-28
DO - 10.1007/978-3-211-35205-2-28
M3 - Chapter
C2 - 17370782
SN - 9783211352045
T3 - Acta Neurochirurgica, Supplementum
SP - 145
EP - 150
BT - Advances in Functional and Reparative Neurosurgery
PB - Springer-Verlag Wien
ER -