TY - CHAP
T1 - A new and simple transection knife for study of neurodegeneration and neuroregeneration in animal model
AU - Park, J. Y.
AU - Kim, S. D.
AU - Park, J.
AU - Lim, D. J.
AU - Lee, H. K.
AU - Chung, H. S.
PY - 2006
Y1 - 2006
N2 - The purpose of this study was to design and make a simple, inexpensive brain knife that could produce consistent results following transection in animal model. After testing various materials including commercially available products, microelectrode recording needles as used in deep brain stimulation (DBS) surgery were selected as ideal candidates. They were modified to serve as type of wire-knife for the purposes of study. For this study, the major pathway for dopaminergic neuron from substantia nigra to striatum was selected for transection. A total of 40 Sprague-Dawley rats were assigned to 8 groups; normal, 1-4, 6, 8, and 10 weeks post-transection. Degree of cell death was determined and surviving neurons were counted by means of fluorescent microscopic examination, immunohistochemistry involving tyrosine hydroxylase (TH)- immunoreactive staining, and mapping to verify complete transection. Compared to control, percentage of remaining neurons in each group was 61.3, 36.8, 29.9, 5.1, 5.9, 7.0%, respectively. Completeness of lesion was correlated with the absence of TH-immunoreactivity in the striatum. Our model seems to provide complete cell death in early period after transection with consistent results. Thus, this type of brain knife can be very handy, without any extra cost, in any research model involving transection of fiber bundle for studies on neurodegeneration and neuroregeneration.
AB - The purpose of this study was to design and make a simple, inexpensive brain knife that could produce consistent results following transection in animal model. After testing various materials including commercially available products, microelectrode recording needles as used in deep brain stimulation (DBS) surgery were selected as ideal candidates. They were modified to serve as type of wire-knife for the purposes of study. For this study, the major pathway for dopaminergic neuron from substantia nigra to striatum was selected for transection. A total of 40 Sprague-Dawley rats were assigned to 8 groups; normal, 1-4, 6, 8, and 10 weeks post-transection. Degree of cell death was determined and surviving neurons were counted by means of fluorescent microscopic examination, immunohistochemistry involving tyrosine hydroxylase (TH)- immunoreactive staining, and mapping to verify complete transection. Compared to control, percentage of remaining neurons in each group was 61.3, 36.8, 29.9, 5.1, 5.9, 7.0%, respectively. Completeness of lesion was correlated with the absence of TH-immunoreactivity in the striatum. Our model seems to provide complete cell death in early period after transection with consistent results. Thus, this type of brain knife can be very handy, without any extra cost, in any research model involving transection of fiber bundle for studies on neurodegeneration and neuroregeneration.
KW - Knife
KW - cell death
KW - degeneration
KW - electrode
KW - substantia nigra
KW - transection
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U2 - 10.1007/978-3-211-35205-2_28
DO - 10.1007/978-3-211-35205-2_28
M3 - Chapter
C2 - 17370782
AN - SCOPUS:84872513767
SN - 9783211352045
T3 - Acta Neurochirurgica, Supplementum
SP - 145
EP - 150
BT - Advances in Functional and Reparative Neurosurgery
PB - Springer-Verlag Wien
ER -