Abstract
A new spin filter consisting of 50 μm (nominal pore size) depth filters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.
Original language | English |
---|---|
Pages (from-to) | 406-410 |
Number of pages | 5 |
Journal | Journal of microbiology and biotechnology |
Volume | 12 |
Issue number | 3 |
Publication status | Published - 2002 |
Bibliographical note
Copyright:Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
Keywords
- Centrifugal force
- High density culture
- Pivotal movement
- Spin filter
- Yeast
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology