A novel alkaline lipase from Ralstonia with potential application in biodiesel production

Hah Young Yoo, Jaya Ram Simkhada, Seung Sik Cho, Don Hee Park, Seung Wook Kim, Chi Nam Seong, Jin Cheol Yoo

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)


With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000Da by SDS-PAGE. The activity was highest at 50-55°C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45°C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and Km and Vmax for p-nitrophenyl palmitate were 2.73±0.6mM and 101.4±1.9mM/minmg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content.

Original languageEnglish
Pages (from-to)6104-6111
Number of pages8
JournalBioresource technology
Issue number10
Publication statusPublished - 2011 May

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation (KRF) grant funded by the Korean government (MEST) ( 2010-0029178 ) and Technology Development Program for Agriculture and Forestry , Ministry of Agriculture and Forestry , Republic of Korea, 2007 ( 307014-5 ).


  • Biodiesel production
  • Lipase
  • Purification
  • Ralstonia sp. CS274

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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