A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell

Hyung Soo Park; Byung Hong Kim; Hyo Suk Kim; Hyung Joo Kim; Gwang Tae Kim; Mia Kim; In Seop Chang; Yong Keun Park; Hyo Ihl Chang

doi:10.1006/anae.2001.0399

A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell

Hyung Soo Park, Byung Hong Kim, Hyo Suk Kim, Hyung Joo Kim, Gwang Tae Kim, Mia Kim, In Seop Chang, Yong Keun Park, Hyo Ihl Chang

Research output: Contribution to journal › Article › peer-review

500 Citations (Scopus)

Abstract

An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 μm long, 0.5-0.6 μm wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37°C and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO₂ and H₂. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria.

Original language	English
Pages (from-to)	297-306
Number of pages	10
Journal	Anaerobe
Volume	7
Issue number	6
DOIs	https://doi.org/10.1006/anae.2001.0399
Publication status	Published - 2001

Bibliographical note

Funding Information:
We are deeply grateful to Professor Julian Wimpenny, Mr Michael Turner and Mr Chang-Suk Lee for their assistance in electron microscopy. This work was supported by the Ministry of Science and Technology, Korea (Grant No. 99-N1-01-01-A-03 and 00-J-BP-B-03).

Keywords

Clostridium sp. EG3
Cyclic voltammogram
Dissimilatory Fe(III) reduction
Electrochemically active bacteria
Microbial fuel cell

ASJC Scopus subject areas

Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/anae.2001.0399

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title = "A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell",

abstract = "An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 μm long, 0.5-0.6 μm wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37°C and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2 and H2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria.",

keywords = "Clostridium sp. EG3, Cyclic voltammogram, Dissimilatory Fe(III) reduction, Electrochemically active bacteria, Microbial fuel cell",

author = "Park, {Hyung Soo} and Kim, {Byung Hong} and Kim, {Hyo Suk} and Kim, {Hyung Joo} and Kim, {Gwang Tae} and Mia Kim and Chang, {In Seop} and Park, {Yong Keun} and Chang, {Hyo Ihl}",

note = "Funding Information: We are deeply grateful to Professor Julian Wimpenny, Mr Michael Turner and Mr Chang-Suk Lee for their assistance in electron microscopy. This work was supported by the Ministry of Science and Technology, Korea (Grant No. 99-N1-01-01-A-03 and 00-J-BP-B-03).",

year = "2001",

doi = "10.1006/anae.2001.0399",

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volume = "7",

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journal = "Anaerobe",

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T1 - A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell

AU - Park, Hyung Soo

AU - Kim, Byung Hong

AU - Kim, Hyo Suk

AU - Kim, Hyung Joo

AU - Kim, Gwang Tae

AU - Kim, Mia

AU - Chang, In Seop

AU - Park, Yong Keun

AU - Chang, Hyo Ihl

N1 - Funding Information: We are deeply grateful to Professor Julian Wimpenny, Mr Michael Turner and Mr Chang-Suk Lee for their assistance in electron microscopy. This work was supported by the Ministry of Science and Technology, Korea (Grant No. 99-N1-01-01-A-03 and 00-J-BP-B-03).

PY - 2001

Y1 - 2001

N2 - An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 μm long, 0.5-0.6 μm wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37°C and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2 and H2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria.

AB - An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 μm long, 0.5-0.6 μm wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37°C and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2 and H2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria.

KW - Clostridium sp. EG3

KW - Cyclic voltammogram

KW - Dissimilatory Fe(III) reduction

KW - Electrochemically active bacteria

KW - Microbial fuel cell

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A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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