A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana

Yun Young Kim; Kwang Wook Jung; Ji Ung Jeung; Jeong Sheop Shin

doi:10.1016/j.jplph.2011.09.006

A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana

Yun Young Kim, Kwang Wook Jung, Ji Ung Jeung, Jeong Sheop Shin

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.

Original language	English
Pages (from-to)	212-216
Number of pages	5
Journal	Journal of Plant Physiology
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/j.jplph.2011.09.006
Publication status	Published - 2012 Jan 15

Bibliographical note

Funding Information:
This research was supported by a grant ( 2011-0015848 to J.S.S.) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and grants ( PJ008198 to J.S.S. and PJ008160 to J.S.S.) from the Next-Generation BioGreen21 Program funded by the Rural Development Administration , and also partially supported by a Korea University grant, Republic of Korea .

Keywords

ASK
Anther dehiscence
Arabidopsis
Endothecial secondary wall thickening
F-box protein

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/j.jplph.2011.09.006

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title = "A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana",

abstract = "In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.",

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note = "Funding Information: This research was supported by a grant ( 2011-0015848 to J.S.S.) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and grants ( PJ008198 to J.S.S. and PJ008160 to J.S.S.) from the Next-Generation BioGreen21 Program funded by the Rural Development Administration , and also partially supported by a Korea University grant, Republic of Korea . ",

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N1 - Funding Information: This research was supported by a grant ( 2011-0015848 to J.S.S.) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and grants ( PJ008198 to J.S.S. and PJ008160 to J.S.S.) from the Next-Generation BioGreen21 Program funded by the Rural Development Administration , and also partially supported by a Korea University grant, Republic of Korea .

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N2 - In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.

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A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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