In plants, the regulation of protein turnover by the ubiquitin proteasome system (UPS) is a key posttranslational mechanism underlying diverse cellular processes. However, the participation of the UPS in cellular processes involved in anther dehiscence, especially endothecial secondary wall (ESW) thickening, has not been characterized. Here, we report that a novel F-box protein in arabidopsis, designated SAF1 (Secondary wall thickening-Associated F-box 1), negatively regulates ESW thickening in the anther. SAF1 is predominantly expressed in flower tissues and interacts with Arabidopsis-Skp1-like 19 (ASK19). SAF1-overexpressed (Ox) lines showed reduced fertility due to a lack or loss of ESW thickening in the anther and inhibition of the expression of relevant genes, such as IRREGURAR XYLEMs (IRXs) in flowers. These findings suggest that the novel Skp/Cul/F-box (SCF) complex consisting of SAF1 as an F-box protein and ASK19 as a Skp functions in secondary wall thickening of the anther endothecium.
Bibliographical noteFunding Information:
This research was supported by a grant ( 2011-0015848 to J.S.S.) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and grants ( PJ008198 to J.S.S. and PJ008160 to J.S.S.) from the Next-Generation BioGreen21 Program funded by the Rural Development Administration , and also partially supported by a Korea University grant, Republic of Korea .
- Anther dehiscence
- Endothecial secondary wall thickening
- F-box protein
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science