A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

Kyochan Kim; Eun Jung Kim; Byung Gon Ryu; Soojung Park; Yoon E. Choi; Ji Won Yang

doi:10.1016/j.biortech.2012.10.139

A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

Kyochan Kim, Eun Jung Kim, Byung Gon Ryu, Soojung Park, Yoon E. Choi, Ji Won Yang

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or division, whereas carbon was necessary for the entire process of cell development, particularly cell enlargement during late stages. Using these distinctive yield coefficients, a modified fed-batch cultivation method was designed, resulting in increases in palmitic acid (PA) and DHA production of up to 137% and 29%, respectively. This modified fed-batch cultivation method, using appropriate supplies of nitrogen and carbon, may improve the yields of PA and DHA, thus expanding the biotechnological applications of Aurantiochytrium sp. KRS101.

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	Bioresource technology
Volume	135
DOIs	https://doi.org/10.1016/j.biortech.2012.10.139
Publication status	Published - 2013 May
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ). We thank Dr. Chul-Ho Kim at Korea Research Institute of Bioscience and Biotechnology (KRIBB) for providing the Aurantiochytrium sp. KRS101.

Keywords

Aurantiochytrium sp.
Biodiesel
Docosahexaenoic acid
Modified fed-batch
Yield coefficients

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2012.10.139

Cite this

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title = "A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid",

abstract = "The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or division, whereas carbon was necessary for the entire process of cell development, particularly cell enlargement during late stages. Using these distinctive yield coefficients, a modified fed-batch cultivation method was designed, resulting in increases in palmitic acid (PA) and DHA production of up to 137% and 29%, respectively. This modified fed-batch cultivation method, using appropriate supplies of nitrogen and carbon, may improve the yields of PA and DHA, thus expanding the biotechnological applications of Aurantiochytrium sp. KRS101.",

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author = "Kyochan Kim and {Jung Kim}, Eun and Ryu, {Byung Gon} and Soojung Park and Choi, {Yoon E.} and Yang, {Ji Won}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ). We thank Dr. Chul-Ho Kim at Korea Research Institute of Bioscience and Biotechnology (KRIBB) for providing the Aurantiochytrium sp. KRS101.",

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doi = "10.1016/j.biortech.2012.10.139",

language = "English",

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AU - Ryu, Byung Gon

AU - Park, Soojung

AU - Choi, Yoon E.

AU - Yang, Ji Won

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ). We thank Dr. Chul-Ho Kim at Korea Research Institute of Bioscience and Biotechnology (KRIBB) for providing the Aurantiochytrium sp. KRS101.

PY - 2013/5

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AB - The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or division, whereas carbon was necessary for the entire process of cell development, particularly cell enlargement during late stages. Using these distinctive yield coefficients, a modified fed-batch cultivation method was designed, resulting in increases in palmitic acid (PA) and DHA production of up to 137% and 29%, respectively. This modified fed-batch cultivation method, using appropriate supplies of nitrogen and carbon, may improve the yields of PA and DHA, thus expanding the biotechnological applications of Aurantiochytrium sp. KRS101.

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KW - Docosahexaenoic acid

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KW - Yield coefficients

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A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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