Abstract
A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.
Original language | English |
---|---|
Pages (from-to) | 154-160 |
Number of pages | 7 |
Journal | Analytica Chimica Acta |
Volume | 1050 |
DOIs | |
Publication status | Published - 2019 Mar 7 |
Bibliographical note
Funding Information:We acknowledge financial support from the National Key Project of China ( 2017YFC0908600 ), the National Natural Science Foundation of China (Grant 21405136 ), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China ( LY18H300003 ), Scientific Research Fund of Zhejiang Provincial Education Department ( N20140206 ). We thank Ms. Haihong Hu for managing instruments and helping experiments.
Funding Information:
We acknowledge financial support from the National Key Project of China (2017YFC0908600), the National Natural Science Foundation of China (Grant 21405136), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China (LY18H300003), Scientific Research Fund of Zhejiang Provincial Education Department (N20140206). We thank Ms. Haihong Hu for managing instruments and helping experiments.
Publisher Copyright:
© 2018 Elsevier B.V.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
Keywords
- Helper qPCR platform
- Platinum detection
- Platinum-responsive on/off switch
- Pt-DNA coordination
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy