A novel helper qPCR system for platinum detection via Pt-DNA coordination

Sheng Cai; Dan Yang; Xueke Tian; Jiawei Ye; Mingcheng Xu; Abdu Ahmed Abdullah AL-maskri; Cheulhee Jung; Su Zeng

doi:10.1016/j.aca.2018.11.007

A novel helper qPCR system for platinum detection via Pt-DNA coordination

Sheng Cai, Dan Yang, Xueke Tian, Jiawei Ye, Mingcheng Xu, Abdu Ahmed Abdullah AL-maskri, Cheulhee Jung, Su Zeng

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.

Original language	English
Pages (from-to)	154-160
Number of pages	7
Journal	Analytica Chimica Acta
Volume	1050
DOIs	https://doi.org/10.1016/j.aca.2018.11.007
Publication status	Published - 2019 Mar 7

Bibliographical note

Funding Information:
We acknowledge financial support from the National Key Project of China ( 2017YFC0908600 ), the National Natural Science Foundation of China (Grant 21405136 ), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China ( LY18H300003 ), Scientific Research Fund of Zhejiang Provincial Education Department ( N20140206 ). We thank Ms. Haihong Hu for managing instruments and helping experiments.

Funding Information:
We acknowledge financial support from the National Key Project of China (2017YFC0908600), the National Natural Science Foundation of China (Grant 21405136), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China (LY18H300003), Scientific Research Fund of Zhejiang Provincial Education Department (N20140206). We thank Ms. Haihong Hu for managing instruments and helping experiments.

Publisher Copyright:
© 2018 Elsevier B.V.

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

Helper qPCR platform
Platinum detection
Platinum-responsive on/off switch
Pt-DNA coordination

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/j.aca.2018.11.007

Cite this

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title = "A novel helper qPCR system for platinum detection via Pt-DNA coordination",

abstract = "A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.",

keywords = "Helper qPCR platform, Platinum detection, Platinum-responsive on/off switch, Pt-DNA coordination",

author = "Sheng Cai and Dan Yang and Xueke Tian and Jiawei Ye and Mingcheng Xu and {Abdullah AL-maskri}, {Abdu Ahmed} and Cheulhee Jung and Su Zeng",

note = "Funding Information: We acknowledge financial support from the National Key Project of China ( 2017YFC0908600 ), the National Natural Science Foundation of China (Grant 21405136 ), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China ( LY18H300003 ), Scientific Research Fund of Zhejiang Provincial Education Department ( N20140206 ). We thank Ms. Haihong Hu for managing instruments and helping experiments. Funding Information: We acknowledge financial support from the National Key Project of China (2017YFC0908600), the National Natural Science Foundation of China (Grant 21405136), a Korea University Grant, the Zhejiang Provincial Natural Science Foundation of China (LY18H300003), Scientific Research Fund of Zhejiang Provincial Education Department (N20140206). We thank Ms. Haihong Hu for managing instruments and helping experiments. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

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AU - Ye, Jiawei

AU - Xu, Mingcheng

AU - Abdullah AL-maskri, Abdu Ahmed

AU - Jung, Cheulhee

AU - Zeng, Su

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PY - 2019/3/7

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N2 - A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.

AB - A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples.

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A novel helper qPCR system for platinum detection via Pt-DNA coordination

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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