A novel reflectance-based aptasensor using gold nanoparticles for the detection of oxytetracycline

Ho Bin Seo, Young Seop Kwon, Ji Eun Lee, David Cullen, Hongseok Noh, Man Bock Gu

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    We present a novel reflectance-based colorimetric aptasensor using gold nanoparticles for the detection of oxytetracycline for the first time. It was found that the reflectance-based measurement at two wavelengths (650 and 520 nm) can generate more stable and sensitive signals than absorbance-based sensors to determine the aggregation of AuNPs, even at high AuNP concentrations. One of the most common antibacterial agents, oxytetracycline (OTC), was detected at concentrations as low as 1 nM in both buffer solution and tap water, which was 25-fold more sensitive, compared to the previous absorbance-based colorimetric aptasensors. This reflectance-based colorimetric aptasensor using gold nanoparticles is considered to be a better platform for portable sensing of small molecules using aptamers.

    Original languageEnglish
    Pages (from-to)6671-6675
    Number of pages5
    JournalAnalyst
    Volume140
    Issue number19
    DOIs
    Publication statusPublished - 2015 Oct 7

    Bibliographical note

    Publisher Copyright:
    © The Royal Society of Chemistry 2015.

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy
    • Electrochemistry

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