TY - JOUR
T1 - A performance improvement of enzyme-based electrochemical lactate sensor fabricated by electroplating novel PdCu mediator on a laser induced graphene electrode
AU - Han, Ji Hoon
AU - Hyun Park, Sang
AU - Kim, Saeyoung
AU - Jungho Pak, James
N1 - Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1A2B 4009088, “Development of IoT-based wearable multi-sensor for human sweat analysis using laser-induced graphene electrode”).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/12
Y1 - 2022/12
N2 - A lactate sensor for lactate sensing using porous laser-induced graphene (LIG) electrodes with an electrodeposited PdCu catalyst was developed in this study. CO2 laser was used to convert the polyimide film surface to multilayered LIG. The morphology and composition of LIG were analyzed through field-emission scanning electron microscopy and Raman spectroscopy, respectively, to confirm that the fabricated LIG electrode was composed of porous and stacked graphene layers. PdCu was electrodeposited on the LIG electrode and lactate oxidase (LOx) was immobilized on the LIG surface to create a LOx/PdCu/LIG structure. According to the Randles–Ševčík equation, the calculated active surface area of the fabricated PdCu/LIG electrode was ∼12.8 mm2, which was larger than the apparent area of PdCu/LIG (1.766 mm2) by a factor of 7.25. The measured sensitivities of the fabricated lactate sensors with the LOx/PdCu/LIG electrode were −51.91 μA/mM·cm2 (0.1–5 mM) and −17.18 μA/mM·cm2 (5–30 mM). The calculated limit of detection was 0.28 μM. The selectivity of the fabricated lactate sensor is excellent toward various potentially interfering materials such as ascorbic acid, uric acid, lactose, sucrose, K+ and Na+.
AB - A lactate sensor for lactate sensing using porous laser-induced graphene (LIG) electrodes with an electrodeposited PdCu catalyst was developed in this study. CO2 laser was used to convert the polyimide film surface to multilayered LIG. The morphology and composition of LIG were analyzed through field-emission scanning electron microscopy and Raman spectroscopy, respectively, to confirm that the fabricated LIG electrode was composed of porous and stacked graphene layers. PdCu was electrodeposited on the LIG electrode and lactate oxidase (LOx) was immobilized on the LIG surface to create a LOx/PdCu/LIG structure. According to the Randles–Ševčík equation, the calculated active surface area of the fabricated PdCu/LIG electrode was ∼12.8 mm2, which was larger than the apparent area of PdCu/LIG (1.766 mm2) by a factor of 7.25. The measured sensitivities of the fabricated lactate sensors with the LOx/PdCu/LIG electrode were −51.91 μA/mM·cm2 (0.1–5 mM) and −17.18 μA/mM·cm2 (5–30 mM). The calculated limit of detection was 0.28 μM. The selectivity of the fabricated lactate sensor is excellent toward various potentially interfering materials such as ascorbic acid, uric acid, lactose, sucrose, K+ and Na+.
KW - Electrochemical sensor
KW - Enzymatic sensor
KW - Lactate sensor
KW - PdCu
KW - Porous laser-induced graphene
KW - Ultra sensitivity
UR - http://www.scopus.com/inward/record.url?scp=85138471266&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2022.108259
DO - 10.1016/j.bioelechem.2022.108259
M3 - Article
C2 - 36179392
AN - SCOPUS:85138471266
SN - 1567-5394
VL - 148
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
M1 - 108259
ER -