A Portable Diagnostic Assay, Genetic Diversity, and Isolation of Seoul Virus from Rattus norvegicus Collected in Gangwon Province, Republic of Korea

Kyungmin Park, Seung Ho Lee, Jongwoo Kim, Jingyeong Lee, Geum Young Lee, Seungchan Cho, Juyoung Noh, Jeewan Choi, Juwon Park, Dong Hyun Song, Se Hun Gu, Hyeongseok Yun, Jung Eun Kim, Daesang Lee, Il Ung Hwang, Won Keun Kim, Jin Won Song

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Seoul virus (SEOV), an etiological agent for hemorrhagic fever with renal syndrome, poses a significant public health threat worldwide. This study evaluated the feasibility of a mobile Biomeme platform for facilitating rapid decision making of SEOV infection. A total of 27 Rattus norvegicus were collected from Seoul Metropolitan City and Gangwon Province in Republic of Korea (ROK), during 2016–2020. The serological and molecular prevalence of SEOV was 5/27 (18.5%) and 2/27 (7.4%), respectively. SEOV RNA was detected in multiple tissues of rodents using the Biomeme device, with differences in Ct values ranging from 0.6 to 2.1 cycles compared to a laboratory benchtop system. Using amplicon-based next-generation sequencing, whole-genome sequences of SEOV were acquired from lung tissues of Rn18-1 and Rn19-5 collected in Gangwon Province. Phylogenetic analysis showed a phylogeographical diversity of rat-borne orthohantavirus collected in Gangwon Province. We report a novel isolate of SEOV Rn19-5 from Gangwon Province. Our findings demonstrated that the Biomeme system can be applied for the molecular diagnosis of SEOV comparably to the laboratory-based platform. Whole-genome sequencing of SEOV revealed the phylogeographical diversity of orthohantavirus in the ROK. This study provides important insights into the field-deployable diagnostic assays and genetic diversity of orthohantaviruses for the rapid response to hantaviral outbreaks in the ROK.

Original languageEnglish
Article number1047
Issue number9
Publication statusPublished - 2022 Sept

Bibliographical note

Funding Information:
This study was supported by the Agency for Defense Development (UE202026GD) and the Institute for Basic Science Korea (IBS-R801-D92022-a03). In addition, the research was a project titled “Diagnosis, treatment and control technology based on big data of infectious virus in the marine environment” funded by the Korea Institute of Marine Science & Technology Promotion (Ref. No. 21210466) of the Ministry of Oceans and Fisheries (MOF) of South Korea and a project titled “Development of One-health monitoring and active surveillance of novel emerging zoonotic RNA viruses” funded by Basic Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2021R1I1A2049607), respectively.

Publisher Copyright:
© 2022 by the authors.


  • Biomeme system
  • Seoul virus
  • genetic diversity
  • hantavirus isolation
  • hemorrhagic fever with renal syndrome
  • molecular diagnosis
  • next-generation sequencing
  • portable diagnostic assay
  • whole-genome sequencing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • General Immunology and Microbiology
  • Microbiology (medical)
  • Infectious Diseases


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